Ludwig Maximilians University

2 ARTICLES PUBLISHED IN JoVE

Behavior

Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice

Alessandro F. Ulivi¹, Tim P. Castello-Waldow¹, Ghabiba Weston^1,2, Long Yan³, Ryohei Yasuda³, Alon Chen^1,4, Alessio Attardo¹

¹Dept. of Stress Neurobiology and Neurogenetics, Max Planck Institute of Psychiatry, ²Graduate School of Systemic Neurosciences, Ludwig Maximilians University, ³Max Planck Florida Institute for Neuroscience, ⁴Dept. of Neurobiology, Weizmann Institute of Science

This method describes a chronic preparation that allows optical access to the hippocampus of living mice. This preparation can be used to perform longitudinal optical imaging of neuronal structural plasticity and activity-evoked cellular plasticity over a period of several weeks.

Immunology and Infection

Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

Hilger Jagau^1,2, Ina-Kristin Behrens^1,3, Michael Steinert^1,4, Simone Bergmann¹

¹Institut für Mikrobiologie, Technische Universität Braunschweig, ²Devision of Infection Medicine, Department of Clinical Science, Lund University, ³Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Ludwig Maximilians University, ⁴Department of Molecular Infection Biology, Helmholtz Center for Infection Research

This study describes the microscopic monitoring of pneumococcus adherence to von Willebrand factor strings produced on the surface of differentiated human primary endothelial cells under shear stress in defined flow conditions. This protocol can be extended to detailed visualization of specific cell structures and quantification of bacteria by applying differential immunostaining procedures.