Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

6.3K Views

•

09:34 min

•

October 31st, 2019

DOI :

10.3791/60323-v

October 31st, 2019

•

Hilger Jagau¹^,², Ina-Kristin Behrens¹^,³, Michael Steinert¹^,⁴, Simone Bergmann¹

¹Institut für Mikrobiologie, Technische Universität Braunschweig, ²Devision of Infection Medicine, Department of Clinical Science, Lund University, ³Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Ludwig Maximilians University, ⁴Department of Molecular Infection Biology, Helmholtz Center for Infection Research

Summary

Explore More Videos

Pneumococcus Infection

Primary Human Endothelial Cells

Bacterial Infection Processes

Vascular Integrity

Morphogenesis Mechanisms

Inflammation Responses

Immune Regulation

Cell Culture Handling

Endothelial Cell Differentiation

Chapters in this video

0:04

Title

1:29

Endothelial Cell Cultivation of HUVEC, Induction of VWF (Von Willebrand Factor)-release and Visualization of Multimerized VWF Strings

5:53

Microscopic Evaluation of Bacterial Attachment to VWF-strings in Flow in Real Time and after Sample Fixation

7:12

Results: Analyses of Bacterial Attachment to VWF Strings

8:33

Conclusion

Related Videos

14:01

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

14.4K Views

10:09

Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells

19.0K Views

08:54

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

17.0K Views

10:02

piggyBac Transposon System Modification of Primary Human T Cells

17.2K Views

14:48

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

26.2K Views

11:22

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

17.1K Views

08:14

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

12.3K Views

10:15

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

7.8K Views

09:09

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

23.4K Views

08:07

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

10.6K Views