Sign In

New York University College of Dentistry

3 ARTICLES PUBLISHED IN JoVE

image

Medicine

Technique to Collect Fungiform (Taste) Papillae from Human Tongue
Andrew I. Spielman *1, M. Yanina Pepino 2, Roy Feldman 3,4,5, Joseph G. Brand *4,6
1Department of Basic Science and Craniofacial Biology, College of Dentistry, New York University, 2Department of Internal Medicine and Department of Psychiatry, School of Medicine, Washington University in St. Louis, 3Veterans Affairs Medical Center, 4School of Dental Medicine, Department of Biochemistry, University of Pennsylvania-School of Medicine, 5Monell Chemical Senses Center, 6Monell Chemical Senses Center

Knowledge of molecular mechanisms underlying gustatory transduction has recently enjoyed significant advances, largely due to using animal models. However, the wide diversity in taste sensitivity and specificity among mammals warrants studies in human tissue. We describe a biopsy technique to collect living taste cells from the papillae on human tongue.

image

Neuroscience

Isolation and Culture of Human Fungiform Taste Papillae Cells
Hakan Ozdener 1, Andrew I. Spielman 2, Nancy E. Rawson 3
1Monell Chemical Senses Center, 2New York University College of Dentistry, 3AFB International

We aimed to develop a reproducible protocol for isolating and maintaining long-term cultures of human fungiform taste papillae cells. Cells from human fungiform papillae obtained by biopsy were successfully maintained in culture for more than eight passages (12 months) without loss of viability.

image

Immunology and Infection

Zika Virus Specific Diagnostic Epitope Discovery
Maite Sabalza 1, Cheryl A. Barber 1, William R. Abrams 1, Richard Montagna 2, Daniel Malamud 1,3
1Department of Basic Sciences and Craniofacial Development, New York University College of Dentistry, 2Rheonix, Inc., 3Department of Medicine, New York University Langone School of Medicine

In this protocol, we describe a technique to discover Zika virus specific diagnostic peptides using a high-density peptide microarray. This protocol can readly be adapted for other emerging infectious diseases.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved