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SUNY Oswego

3 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions
Melinda L. Koyle 1, Madeline Veloz 1, Alec M. Judd 1, Adam C.-N. Wong 2,4, Peter D. Newell 2,5, Angela E. Douglas 2,3, John M. Chaston 1,2
1Department of Plant and Wildlife Sciences, Brigham Young University, 2Department of Entomology, Cornell University, 3Department of Molecular Biology and Genetics, Cornell University, 4Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, 5Biological Sciences, SUNY Oswego

A method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions is presented. Fly embryos are dechorionated in sodium hypochlorite, transferred aseptically to sterile diet, and reared in closed containers. Inoculating diet and embryos with bacteria leads to gnotobiotic associations, and bacterial presence is confirmed by plating whole-body Drosophila homogenates.

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Biology

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
Yulia Artemenko 1, Peter N. Devreotes 2
1Department of Biological Sciences, SUNY Oswego, 2Department of Cell Biology, Johns Hopkins University School of Medicine

Here we describe methods for assessing cellular response to acute mechanical stimulation. In the microscopy-based assay, we examine localization of fluorescently-labeled biosensors following brief stimulation with shear flow. We also test activation of various proteins of interest in response to acute mechanical stimulation biochemically.

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Environment

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy
Jessica Gibbons 1, Poongodi Geetha-Loganathan 1
1Department of Biological Sciences, SUNY Oswego

Here, we present detailed processing protocols for imaging delicate tissue samples using scanning electron microscopy (SEM). Three different processing methods, namely, hexamethyl disilazana (HMDS) chemical drying, simple air drying, and critical point drying are described for preparing rigid eggshells, embryos at early developmental stages, and fungal cultures respectively.

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