Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

20.9K Views

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09:34 min

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July 30th, 2016

DOI :

10.3791/54219-v

July 30th, 2016

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Melinda L. Koyle¹, Madeline Veloz¹, Alec M. Judd¹, Adam C.-N. Wong²^,⁴, Peter D. Newell²^,⁵, Angela E. Douglas²^,³, John M. Chaston¹^,²

¹Department of Plant and Wildlife Sciences, Brigham Young University, ²Department of Entomology, Cornell University, ³Department of Molecular Biology and Genetics, Cornell University, ⁴Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, ⁵Biological Sciences, SUNY Oswego

Summary

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Keywords Drosophila Melanogaster

Chapters in this video

0:05

Title

0:43

Bacterial Culture and Diet Preparation

2:16

Egg-laying Cages

3:13

Egg Collection

3:55

Egg Dechorionation and Sterile Diet Transfer

5:33

Gnotobiotic Fly Generation

6:40

Measuring CFU Load and Testing for Sterility

8:05

Results: Successful Axenic Fly Rearing Confirmed with CFUs

8:49

Conclusion

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