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Kanazawa University

7 ARTICLES PUBLISHED IN JoVE

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Biology

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
Karthika Natarajan 1,2, Yi Xie 1,3, Takeo Nakanishi 4, Rebecca S. Moreci 5,6, Pancharatnam Jeyasuria 7, Arif Hussain 1,3,8,9, Douglas D. Ross 1,3,8,9,10,11
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Pharmaceutical Sciences, University of Maryland School of Pharmacy, 3Baltimore VA Medical Center, 4Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences, Kanazawa University, 5Obstetrics, Gynecology and Reproductive Science, University of Pittsburgh, 6Magee Women's Research Institute, 7Obstetrics, Gynecology, Perinatal Research Branch (NICHD), Wayne State University School of Medicine, 8Medicine, University of Maryland School of Medicine, 9Pathology, University of Maryland School of Medicine, 10Pharmacology, University of Maryland School of Medicine, 11Experimental Therapeutics, University of Maryland School of Medicine

With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.

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Medicine

A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device
Kazuhiro Ogai 1, Masakazu Fukuoka 2, Kei-ichiro Kitamura 3, Kiyoshi Uchide 4, Tetsu Nemoto 3
1Wellness Promotion Science Center, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 2Advanced Research Center for Human Sciences, Waseda University, 3Department of Clinical Laboratory Science, Graduate School of Medical Science, Kanazawa University, 4Asanogawa General Hospital

Recently, we developed a small wireless device for perspiration monitoring. In this article, we present detailed protocols on how to use the device for perspiration monitoring with an example of the sympathetic activity test.

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JoVE Journal

Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos
Saori Nonaka 1, Aki Hori 1, Yoshinobu Nakanishi 1, Takayuki Kuraishi 1
1Graduate School of Medical Sciences, Kanazawa University

We herein describe a phagocytosis assay using the dispersed embryonic cells of Drosophila. It enables us to easily and precisely quantify in vivo phagocytosis levels, and to identify new molecules required for the phagocytosis of apoptotic cells.

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Neuroscience

Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures
Naoyuki Matsumoto 1,2, Nobuhiko Yamamoto 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

This protocol describes a method for simultaneous imaging of thalamocortical axon branching and synapse formation in organotypic cocultures of the thalamus and cerebral cortex. Individual thalamocortical axons and their presynaptic terminals are visualized by a single cell electroporation technique with DsRed and GFP-tagged synaptophysin.

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Neuroscience

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
Shota Kodani *1, Shingo Soya *2, Takeshi Sakurai 2,3
1Department of Molecular Neuroscience and Integrative Physiology, Faculty of Medicine, Kanazawa University, 2International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 3Faculty of Medicine, University of Tsukuba

Here, we describe methods of optogenetic manipulation of particular types of neurons during monitoring of sleep/wakefulness states in mice, presenting our recent work on the bed nucleus of the stria terminalis as an example.

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Education

Investigation into Deep Breathing through Measurement of Ventilatory Parameters and Observation of Breathing Patterns
Masami Yokogawa 1, Tomoyo Kurebayashi 2, Kazuki Soma 3, Hiroichi Miaki 1, Takao Nakagawa 4
1Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 2Rehabilitation Section, Higashimatsuyama Municipal Hospital, 3Division of Health Sciences, Graduate School of Medical Sciences, Kanazawa University, 4Department of Physical and Rehabilitation Medicine, Kanazawa Medical University

Here, we present a protocol to assess two deep breathing patterns of natural and diaphragmatic breathing for their effectiveness and ease of execution. Fifteen participants were selected, utilizing an electrocardiograph and expired gas analyzer for measurement of the ventilatory parameters, together with visual assessment by video capture of thoracoabdominal movement.

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Neuroscience

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation
Nereo Kalebic 1,2, Barbara Langen 1,3, Jussi Helppi 1, Hiroshi Kawasaki 4, Wieland B. Huttner 1
1Max Planck Institute of Molecular Cell Biology and Genetics, 2Human Technopole, 3Landesdirektion Sachsen, 4Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University

Presented here is a protocol to perform genetic manipulation in the embryonic ferret brain using in utero electroporation. This method allows for targeting of neural progenitor cells in the neocortex in vivo.

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