Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

8.0K Views

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11:02 min

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May 27th, 2016

DOI :

10.3791/53827-v

May 27th, 2016

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Karthika Natarajan¹^,², Yi Xie¹^,³, Takeo Nakanishi⁴, Rebecca S. Moreci⁵^,⁶, Pancharatnam Jeyasuria⁷, Arif Hussain¹^,³^,⁸^,⁹, Douglas D. Ross¹^,³^,⁸^,⁹^,¹⁰^,¹¹

¹Greenebaum Cancer Center, University of Maryland School of Medicine, ²Pharmaceutical Sciences, University of Maryland School of Pharmacy, ³Baltimore VA Medical Center, ⁴Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences, Kanazawa University, ⁵Obstetrics, Gynecology and Reproductive Science, University of Pittsburgh, ⁶Magee Women's Research Institute, ⁷Obstetrics, Gynecology, Perinatal Research Branch (NICHD), Wayne State University School of Medicine, ⁸Medicine, University of Maryland School of Medicine, ⁹Pathology, University of Maryland School of Medicine, ¹⁰Pharmacology, University of Maryland School of Medicine, ¹¹Experimental Therapeutics, University of Maryland School of Medicine

Summary

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Keywords Alternative Promoter Usage

Transcriptional Regulation

Chapters in this video

0:05

Title

1:04

In Silico Prediction of Aternative First Exons of Bcrp1 Using BLAST Analysis of Mouse EST Database

4:30

Design of Reporter Constructs for Bcrp1 E1U, E1A, E1B, and E1C Promoters

7:55

Reporter Assay Methods

9:03

Results: Evaluation of Bcrp1 Alternative Promoter Function

10:07

Conclusion

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