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The University of Akron

7 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants
Susan Thompson 1, Jessica Stukel 1, Abrar AlNiemi 1,2, Rebecca Kuntz Willits 1
1Department of Biomedical Engineering, The University of Akron, 2Saint Vincent Saint Mary's High School

This work describes the formation of poly(ethylene glycol) (PEG) microgels via a photopolymerized precipitation reaction. Increasing the PEG molecular weight increased microgel diameter and swelling ratio. Simple adaptations to the PEG microgel precipitation reaction are explored for future applications of microgels as drug delivery vehicles and tissue engineering scaffolds.

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Behavior

Performing Behavioral Tasks in Subjects with Intracranial Electrodes
Matthew A. Johnson 1, Susan Thompson 1, Jorge Gonzalez-Martinez 2, Hyun-Joo Park 1, Juan Bulacio 2, Imad Najm 2, Kevin Kahn 4, Matthew Kerr 4, Sridevi V. Sarma 4, John T. Gale 1,3
1Department of Neurosciences, Cleveland Clinic Foundation, 2Epilepsy Center, Cleveland Clinic Foundation, 3Department of Neurosciences and Center for Neurological Restoration, Cleveland Clinic Foundation, 4Department of Biomedical Engineering, Johns Hopkins University

Patients implanted with intracranial electrodes provide a unique opportunity to record neurological data from multiple areas of the brain while the patient performs behavioral tasks. Here, we present a method of recording from implanted patients that can be reproducible at other institutions with access to this patient population.

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Bioengineering

Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing
Stephanie Lemmo Ham 1, Ehsan Atefi 1, Darcy Fyffe 1, Hossein Tavana 1
1Department of Biomedical Engineering, The University of Akron

A protocol for robotic printing of cancer cell spheroids in a high throughput 96-well plate format using an aqueous two-phase system is presented.

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Neuroscience

Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks
Katelyn B. Sondereker 1, Maureen E. Stabio 2, Jenna R. Jamil 1, Matthew J. Tarchick 1, Jordan M. Renna 1
1Department of Biology, The University of Akron, 2Department of Cell and Developmental Biology, University of Colorado Denver

This protocol provides a comprehensive dissection and analysis guide for the use of deep ocular landmarks, s-opsin immunohistochemistry, Retistruct, and custom code to accurately and reliably orient the isolated mouse retina in anatomical space.

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Education

Application of Design Aspects in Uniaxial Loading Machine Development
Robert P. Thoerner 1, Jonathan D. King 1, Marnie M. Saunders 1
1Department of Biomedical Engineering, University of Akron

Here we present a protocol to develop a pure uniaxial loading machine. Critical design aspects are employed to ensure accurate and reproducible testing results.

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Bioengineering

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling
Sharon L. Truesdell 1, Estee L. George 1, Christopher C. Van Vranken 1, Marnie M. Saunders 1
1Department of Biomedical Engineering, The University of Akron

Here, we present protocols for analyzing bone remodeling within a lab-on-a-chip platform. A 3D printed mechanical loading device can be paired with the platform to induce osteocyte mechanostransduction by deforming the cellular matrix. The platform can also be used to quantify bone remodeling functional outcomes from osteoclasts and osteoblasts (resorption/formation).

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Biology

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT
Janna M. Andronowski 1, Reed A. Davis 1, Caleb W. Holyoke 2
1Department of Biology, The University of Akron, 2Department of Geosciences, The University of Akron

We employed a geological (coring) sampling protocol to procure cortical bone specimens of uniform size for SRµCT experiments from the anterior aspect of human femora. This method is minimally destructive, efficient, results in cylindrical specimens that minimize imaging artifacts from irregular sample shapes and improves microarchitectural visualization and analysis.

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