Winship Cancer Institute of Emory University

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

David R. Myers^*1,2,3,4, Yumiko Sakurai^*1,2,3,4, Reginald Tran^1,2,3,4, Byungwook Ahn^1,2,3,4, Elaissa Trybus Hardy^1,2,3,4, Robert Mannino^1,2,3,4, Ashley Kita^1,2,3,4, Michelle Tsai^1,2,3,4, Wilbur A. Lam^1,2,3,4

¹Department of Pediatrics, Emory University School of Medicine, ²Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ³Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta , ⁴Winship Cancer Institute of Emory University

A method to culture an endothelial cell monolayer throughout the entire inner 3D surface of a microfluidic device with microvascular-sized channels (<30 μm) is described. This in vitro microvasculature model enables the study of biophysical interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.

Medicine

Segmentation and Linear Measurement for Body Composition Analysis using Slice-O-Matic and Horos

Sean Steele¹, Fangyi Lin¹, Thien-Linh Le¹, Alexandra Medline¹, Michelle Higgins¹, Alex Sandberg¹, Sean Evans¹, Gordon Hong², Milton A. Williams³, Mehmet A. Bilen⁴, Sarah Psutka⁵, Kenneth Ogan¹, Viraj A. Master¹

¹Department of Urology, Emory University School of Medicine, ²Northeast Ohio Medical University, ³Department of Urology, University of Alabama at Birmingham, ⁴Department of Medical Oncology, Winship Cancer Institute of Emory University, ⁵Department of Urology, University of Washington

Segmentation and linear measurements quantify skeletal muscle mass and adipose tissues using Computed Tomography and/or Magnetic Resonance Imaging images. Here, we outline the use of Slice-O-Matic software and Horos image viewer for rapid and accurate analysis of body composition. These methods can provide important information for prognosis and risk stratification.