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Stellenbosch University

2 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
Dana M. Freund 1, Katherine A. Sammons 2, Nokwanda P. Makunga 3, Jerry D. Cohen 1, Adrian D. Hegeman 2
1Department of Horticultural Science, Microbial and Plant Genomics Institute, University of Minnesota, 2Department of Horticultural Science, Department of Plant and Microbial Biology, Microbial and Plant Genomics Institute, University of Minnesota, 3Department of Botany and Zoology, Stellenbosch University

Leaf spray mass spectrometry is a direct chemical analysis technique that minimizes the sample preparation and eliminates chromatography, allowing for the rapid detection of small molecules from plant tissues.

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Cancer Research

An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
Shankari Nair 1, Samantha Cairncross 2, Xanthene Miles 1, Monique Engelbrecht 3, Peter du Plessis 1, Julie Bolcaen 1, Randall Fisher 1, Roya Ndimba 1, Charnay Cunningham 4, Wilner Martínez-López 5, Christian Schunck 6, Charlot Vandevoorde 1
1Radiation Biophysics Division, Nuclear Medicine Department, iThemba LABS, 2Department of Biotechnology, University of the Western Cape, 3Department of Medical Biosciences, University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences, Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine, University of the Republic, 6MetaSystems Hard & Software GmbH

This protocol presents the slide preparation and automated scoring of the γ-H2AX foci assay on peripheral blood lymphocytes. To illustrate the method and sensitivity of the assay, isolated lymphocytes were irradiated in vitro. This automated method of DNA DSB detection is useful for fast and high-throughput biological dosimetry applications.

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