Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

8.5K Views

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06:43 min

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June 21st, 2018

DOI :

10.3791/57949-v

June 21st, 2018

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Dana M. Freund¹, Katherine A. Sammons², Nokwanda P. Makunga³, Jerry D. Cohen¹, Adrian D. Hegeman²

¹Department of Horticultural Science, Microbial and Plant Genomics Institute, University of Minnesota, ²Department of Horticultural Science, Department of Plant and Microbial Biology, Microbial and Plant Genomics Institute, University of Minnesota, ³Department of Botany and Zoology, Stellenbosch University

Summary

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Keywords Leaf Spray Mass Spectrometry

Chapters in this video

0:05

Title

0:50

Modification and Preparation of the MS System for Leaf Spray MS

1:38

Preparation of Instrument, Solvents, and Plant Tissue

3:39

Data Quality Assessment and Tandem Mass Fragmentation

5:06

Results: Detection of Mesembrine Alkaloids from Sceletium tortuosum

6:19

Conclusion

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