Chengdu Medical College

6 ARTICLES PUBLISHED IN JoVE

Biology

Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents

Feng Pan^*2, Jie Zhang^*3, Yuyan Liu¹, Liangsheng Lu¹, Xuefeng Qiu⁴, Kangtai Lv⁵, Qipeng Zhang¹

¹State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, Nanjing University, ²Department of Andrology, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, ³School of Pharmaceutical Sciences, Nanjing Tech University, ⁴Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, ⁵Department of Ultrasound, Nanjing Qixia District Maternity and Child Health Care Hospital

Intracavernosal pressure recording (ICP) is an important method to evaluate the erectile function of experimental animals. Here, a detailed protocol is demonstrated for the recording procedure of ICP by catheterizing the crura penis and then electrically stimulating the cavernous nerves in rats.

Medicine

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Di Liu^*1, Xizhu Xu^*2, Yuejin Li², Jie Zhang¹, Xiaoyu Zhang¹, Qihuan Li¹, Haifeng Hou², Dong Li², Wei Wang^1,2,3, Youxin Wang¹

¹Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, ²School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, ³School of Medical and Health Sciences, Edith Cowan University

Immunoglobulin G (IgG) N-glycan is characterized using hydrophilic interaction chromatography UPLC. In addition, the structure of IgG N-glycan is clearly separated. Presented here is an introduction to this experimental method so that it can be widely used in research settings.

JoVE Core

A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice

Jie Zhang^1,2, Fang Fang^1,3, Liang Li¹, Haoliang Huang¹, Hannah C. Webber¹, Yang Sun^1,4, Vinit B. Mahajan^1,4, Yang Hu¹

¹Department of Ophthalmology, Stanford University School of Medicine, ²Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ³Department of Ophthalmology, Second Xiangya Hospital of Central South University, ⁴Department of Ophthalmology, Veterans Affairs Palo Alto Health Care

Here, we present a protocol to induce ocular hypertension and glaucomatous neurodegeneration in mouse eyes by intracameral injection of silicone oil and the procedure for silicone oil removal from the anterior chamber to return elevated intraocular pressure to normal.

Biology

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Jinxi Yuan¹, Jie Zhang¹, Yan Zhang¹, WuYun QiQiGe¹, Wei Liu², Shanchun Yan¹, Guirong Wang²

¹Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, ²Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

This paper presents the step-by-step protocols for CRISPR/Cas9 mutagenesis of the Oriental fruit fly Bactrocera dorsalis. Detailed steps provided by this standardized protocol will serve as a useful guide for generating mutant flies for functional gene studies in B. dorsalis.

Chemistry

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

Fangqing Wang^*1, Zengkai Qian^*1, Guolu Liao^*2, Jing Zeng², Dan Huang², Qiquan Liu², Xingliang Xie¹

¹School of Pharmacy, The Second Class Laboratory of Traditional Chinese Medicine Pharmaceutics, National Administration of Traditional Chinese Medicine, Chengdu Medical College, ²Pengzhou Second People's Hospital

Here, we present a protocol to establish high-performance liquid chromatography (HPLC), coupled with chemical fingerprint multi-pattern recognition, which provides a new strategy for effectively identifying the genuine varieties of Clematidis Armandii Caulis and its adulterants.

Medicine

Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells

Jie Zhang^*1, Yumei Yan^*1, Fang Cheng^*1, Feixiang Huang¹, Xingliang Xie¹, Yanmei Sheng¹

¹School of Pharmacy, Chengdu Medical College

This protocol provides a combination strategy of two herbs to treat injured PC12 cells. The protocol provides a reference for optimizing the best application mode of traditional Chinese medicine (TCM).