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Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells

1.2K Views

10:33 min

November 18th, 2022

DOI :

10.3791/64721-v

November 18th, 2022


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Herb Combination Strategy

Chapters in this video

0:04

Introduction

0:56

Cell Viability Assay

3:02

Screening of Optimal Combination of Two Drugs and its Protective Effect Evaluation

3:37

Annexin V-FITC/PI Assay for Apoptosis Rate

5:00

Immunofluorescence Detection of Caspase-3 Generation

6:16

Immunofluorescence Assay of ROS Level

6:50

Western Blot Detection of Protein Expression of Nrf2, p-Akt, Akt, Bcl-2, and Bax

8:11

Results: Effects of Drug Combinations on the Normal and Injured PC12 Cells

10:01

Conclusion

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