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Chung-Ang University

10 ARTICLES PUBLISHED IN JoVE

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A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries
Gi-Hun Lee 1, Youngjoon Suh 1, Joong Yull Park 1
1School of Mechanical Engineering, College of Engineering, Chung-Ang University

This manuscript introduces a robust method of fabricating concave microwells without the need for complex high-cost facilities. Using magnetic force, steel beads, and a through-hole array, several hundred microwells were formed in a 3 cm x 3 cm polydimethylsiloxane (PDMS) substrate.

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Neuroscience

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
Seth Villarreal 1, Sung Hoon Lee 1,2, Ling-Gang Wu 1
1National Institute of Neurological Disorders and Stroke, 2College of Pharmacy, Chung-ang University

Synaptic vesicle endocytosis is detected by light microscopy of pHluorin fused with synaptic vesicle protein and by electron microscopy of vesicle uptake.

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Bioengineering

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties
Youngmin Seo *1, Chanhwi Park *2, Jaewoo Son 2, Kyungwoo Lee 2, Jangsun Hwang 2, Yeonho Jo 2, Dohyun Lee 2, Muhammad Saad Khan 2, Sachin Ganpat Chavan 2, Yonghyun Choi 2, Dasom Kim 2, Assaf A Gilad 3, Jonghoon Choi 2
1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, 2School of Integrative Engineering, Chung-Ang University, 3Division of Synthetic Biology and Regenerative Medicine, Institute for Quantitative Health Science and Engineering, Michigan State University

In this study, antimicrobial nanomaterials were synthesized by acidic oxidation of multiwalled carbon nanotubes and subsequent reductive deposition of silver nanoparticles. Antimicrobial activity and cytotoxicity tests were performed with the as-prepared nanomaterials.

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Bioengineering

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids
Daehan Kim 1, Gi-Hun Lee 1, Jiheum Park 2, Jung Chan Lee 2, Joong Yull Park 1
1Department of Mechanical Engineering, Graduate School, Chung-Ang University, 2Department of Biomedical Engineering, College of Medicine, Seoul National University

We present a motor-powered centrifugal microfluidic device that can cultivate cell spheroids. Using this device, spheroids of single or multiple cell types could be easily cocultured under high gravity conditions.

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JoVE Core

Elaborate Control of Inkjet Printer for Fabrication of Chip-based Supercapacitors
Seyoung Choi 1, Jihyeon Kang 1, Seohyeon Jang 1, Hojong Eom 1, Ohhyun Kwon 1, Junhyeop Shin 1, Inho Nam 1
1School of Chemical Engineering and Materials Science, Department of Intelligent Energy and Industry, Department of Advanced Materials Engineering, Chung-Ang University

This paper provides a technique for manufacturing chip-based supercapacitors using an inkjet printer. Methodologies are described in detail to synthesize inks, adjust software parameters, and analyze the electrochemical results of the manufactured supercapacitor.

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JoVE Core

Evaluating the Electrochemical Properties of Supercapacitors using the Three-Electrode System
Hojong Eom 1, Jihyeon Kang 1, Seohyeon Jang 1, Ohhyun Kwon 1, Seyoung Choi 1, Junhyeop Shin 1, Inho Nam 1
1School of Chemical Engineering and Materials Science, Department of Intelligent Energy and Industry, Chung-Ang University

The protocol describes the evaluation of various electrochemical properties of supercapacitors using a three-electrode system with a potentiostat device.

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Chemistry

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch
Myeongsu Jeong *1, Jiyoon Park *1, Kwonjung Lee *1, Sunbum Kwon 1
1Department of Chemistry, Chung-Ang University

Photoisomerization quantum yield is a fundamental photophysical property that should be accurately determined in the investigation of newly developed photoswitches. Here, we describe a set of procedures to measure the photoisomerization quantum yield of a photochromic hydrazone as a model bistable photoswitch.

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Engineering

Synthesizing a Gel Polymer Electrolyte for Supercapacitors, Assembling a Supercapacitor Using a Coin Cell, and Measuring Gel Electrolyte Performance
Ohhyun Kwon 1, Jihyeon Kang 1, Seohyeon Jang 1, Seyoung Choi 1, Hojong Eom 1, Junhyeop Shin 1, Jong-Kwon Park 1, Soomin Park 2, Inho Nam 1
1School of Chemical Engineering and Materials Science, Department of Intelligent Energy and Industry, Department of Advanced Materials Engineering, Chung-Ang University, 2School of Energy, Materials and Chemical Engineering, Korea University of Technology and Education

We present a protocol to test the electrochemical and physical properties of a supercapacitor gel polymer electrolyte using a coin cell.

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Bioengineering

Preparation of Zinc Oxide Nanoparticles and the Evaluation of their Antibacterial Effects
Jongjun Park *1, Gahyun Lee *1, Yonghyun Choi 1,2,3, Jiwon Kim 1, Seongeun Park 1, Hee-Young Lee 4, Jonghoon Choi 1,2
1School of Integrative Engineering, Chung-Ang University, 2Feynman Institute of Technology, Nanomedicine Corporation, 3Department of Chemical Science and Engineering, Tokyo Institute of Technology, 4Department of Chemical Engineering, Kumoh National Institute of Technology

In this study, zinc oxide nanoparticles were synthesized using a precipitation method. The antibacterial effect of the synthesized particles was tested against multidrug-resistant methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa bacterial strains.

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Fabrication of Antibacterial Graphene Oxide/Copper Nanocomposites

Fabrication of Antibacterial Graphene Oxide/Copper Nanocomposites
Jiwon Kim 1, Jongjun Park 1, Seongeun Park 1, Yonghyun Choi 1,2,3, Hee-Young Lee 4, Jonghoon Choi 1,2
1School of Integrative Engineering, Chung-Ang University, 2Feynman Institute of Technology, Nanomedicine Corporation, 3Department of Chemical Science and Engineering, Tokyo Institute of Technology, 4Department of Chemical Engineering, Kumoh National Institute of Technology

Herein, we introduce graphene oxide/copper (GO/Cu) nanocomposites as an antibacterial nanomaterial. The antibacterial effectiveness of the GO/Cu nanocomposites was evaluated against both antibiotic-resistant gram-positive and gram-negative bacteria.

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