Sign In

JoVE Journal

Neuroscience

A subscription to JoVE is required to view this content.

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

9.7K Views

07:30 min

September 4th, 2017

September 4th, 2017

9,732 Views

0:05

Title

0:40

Culture Stimulation

1:35

Fixation and Staining

3:42

Epoxy Embedding

5:43

Results: Images of Presynaptic Terminal in Stimulated Neurons with High Potassium

6:49

Conclusion

Transcript

The overall goal of this labeling methodology is to measure synaptic vesicle endocytosis. This method can help answer key questions in the neuroscience field such as the dispersion of formed endocytic vesicles. The main advantage of this technique

Sign in or start your free trial to access this content

Synaptic vesicle endocytosis is detected by light microscopy of pHluorin fused with synaptic vesicle protein and by electron microscopy of vesicle uptake.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More