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University of California San Diego School of Medicine

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Sonia Apaza 1, Susan Espetia 1, Robert H. Gilman 1,2, Sonia Montenegro 3, Susana Pineda 3, Fanny Herhold 1, Romeo Pomari 1, Margaret Kosek 2, Nancy Vu 1, Mayuko Saito 1,2,4
1Laboratorio de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, 2Bloomberg School of Public Health, Johns Hopkins University, 3Laboratorio de Diagnostico Molecular, Facultad de Medicina, University of Concepcion,Chile, 4University of California San Diego School of Medicine

A One-Step RT-PCR assay for detection and genogroup identification of Norovirus isolates from children’s stools, that utilizes primers and TaqMan probes specific to the open reading frame 1 (ORF1)-ORF2 junction region, the most conserved region of the Norovirus genome is described. A non-commercial, cost-effective RNA extraction method is detailed.

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Neuroscience

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis
Sylvia Neumann 1, George E. Campbell *1, Lukasz Szpankowski *2,3, Lawrence S.B. Goldstein 2,4, Sandra E. Encalada 1
1Department of Molecular and Experimental Medicine, Dorris Neuroscience Center, The Scripps Research Institute, 2Department of Cellular and Molecular Medicine, University of California San Diego, 3Department of Bioengineering, University of California San Diego, 4Department of Neurosciences, University of California San Diego School of Medicine

Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor proteins.

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Immunology and Infection

A Murine Model of Group B Streptococcus Vaginal Colonization
Kathryn A. Patras 1, Kelly S. Doran 1,2
1Department of Pediatrics, Division of Host-Microbe Systems & Therapeutics, University of California San Diego School of Medicine, 2Department of Biology and Center for Microbial Sciences, San Diego State University

The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.

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