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Medicine

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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

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15:16 min

July 22nd, 2012

July 22nd, 2012

17,122 Views

0:05

Title

1:37

In-house Silica-guanidinium Method

2:02

Preparation of Silica Suspension for RNA Extraction

3:24

Extraction with Guanidine & Silica

6:08

Commercial Extraction of Viral RNA: QIAamp Viral RNA Kit

8:32

Detection of NoV in Extracted RNA by One-step Real Time PCR with Specific Taqman Probes

14:11

Conclusion

A One-Step RT-PCR assay for detection and genogroup identification of Norovirus isolates from children’s stools, that utilizes primers and TaqMan probes specific to the open reading frame 1 (ORF1)-ORF2 junction region, the most conserved region of the Norovirus genome is described. A non-commercial, cost-effective RNA extraction method is detailed.

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