Akonni Biosystems, Inc.

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

Rebecca C. Holmberg¹, Alissa Gindlesperger¹, Tinsley Stokes¹, Dane Brady², Nitu Thakore³, Philip Belgrader³, Christopher G. Cooney³, Darrell P. Chandler⁴

¹Application Development, Akonni Biosystems, Inc., ²Manufacturing, Akonni Biosystems, Inc., ³Engineering, Akonni Biosystems, Inc., ⁴Research & Development, Akonni Biosystems, Inc.

TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. Consequently, the sample preparation format is compatible with most liquid handling instruments, and can be used for many medium to high-throughput clinical applications and sample types.

Immunology and Infection

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Yvonne Linger¹, Alexander Kukhtin¹, Julia Golova¹, Alexander Perov¹, Peter Qu¹, Christopher Knickerbocker¹, Christopher G. Cooney¹, Darrell P. Chandler¹

¹Akonni Biosystems, Inc.

An amplification microarray combines asymmetric PCR amplification and microarray hybridization into a single chamber, which significantly streamlines microarray workflow for the end user. Simplifying microarray workflow is a necessary first step for creating microarray-based diagnostics that can be routinely used in lower-resource environments.