University of texas Medical Branch at Galveston

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Automated Gait Analysis in Mice with Chronic Constriction Injury

Dong-Wook Kang¹, Jae-Gyun Choi¹, Ji-Young Moon², Suk-Yun Kang², Yeonhee Ryu², Jin Bong Park¹, Hyun-Woo Kim^1,3

¹Department of Physiology and Medical Science, College of Medicine and Brain Research Institute, Chungnam National University, ²KM Fundamental Research Division, Korea Institute of Oriental Medicine (KIOM), ³Department of Neuroscience and Cell Biology, University of Texas Medical Branch at Galveston

The precise assessment of pain response in a neuropathic animal model is critical to investigate the pathophysiology of pain diseases and develop new analgesics. We present a sensitive and objective method to determine the sensory function of the rodent hind paw by an automated gait analysis system.

Immunology and Infection

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Sameera Vipat^*1, Greg Brennan^*1, Chorong Park¹, Sherry L. Haller², Stefan Rothenburg¹

¹Department of Medial Microbiology and Immunology, School of Medicine, University of California Davis, ²Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston

This is a method to generate “scarless” recombinant vaccinia viruses using host-range selection and visual identification of recombinant viruses.

Neuroscience

Burn Injury-Induced Pain and Depression-Like Behavior in Mice

Jae-Gyun Choi^*1, Dong-Wook Kang^*1, Jaehyuk Kim¹, Miae Lee¹, Sheu-Ran Choi², Jin Bong Park¹, Hyun-Woo Kim¹

¹Department of Physiology and Medical Science, College of Medicine and Brain Research Institute, Chungnam National University, ²Department of Pharmacology, Catholic Kwandong University College of Medicine

A transient scald injury (65 °C ± 0.5 °C, 3 s) of one hind paw decreases the threshold (g) to von Frey filament stimulation of the ipsilateral side and alters gait pattern. Besides, burn injury induces depression-like behavior in the forced swimming test.

Immunology and Infection

Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model

Ying Xiao^1,2, Xiaoying S Zhong¹, Xiaowei Liu², Qingjie Li¹

¹Division of Gastroenterology, Department of Internal Medicine, University of Texas Medical Branch at Galveston, ²Department of Gastroenterology, Xiangya Hospital of Central South University

The interaction of genetic susceptibility, mucosal immunity, and intestinal microecological environment is involved in the pathogenesis of inflammatory bowel disease (IBD). In this study, we applied fecal microbiota transplantation to IL-10 deficient mice and investigated its impact on colonic inflammation and heart function.