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Academic Medical Center

4 ARTICLES PUBLISHED IN JoVE

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Medicine

Human Dupuytren's Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions
Sofia Karkampouna 1, Peter Kloen 2, Miryam C. Obdeijn 3, Scott M. Riester 4, Andre J. van Wijnen 4,5, Marianna Kruithof-de Julio 1,6
1Department of Molecular Cell Biology, Cancer Genomics Centre and Centre for Biomedical Genetics, Leiden University Medical Center, 2Department of Orthopedic Surgery, Academic Medical Center, 3Department of Plastic, Reconstructive and Hand Surgery, Academic Medical Center, 4Department of Orthopedic Surgery, Mayo Clinic, 5Department of Biochemistry and Molecular Biology, Mayo Clinic, 6Department of Dermatology, Leiden University Medical Center

Dupuytren’s disease (DD) is a fibroproliferative disease of the palm of the hand. Here, we present a protocol to culture resection specimens from DD in a three-dimensional (3D) culture system. Such short-term culture system allows preservation of the 3D structure and molecular properties of the fibrotic tissue.

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Medicine

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses
Peter G. Wagstaff 1, Abel Swaan 2, Alexandre Ingels 1, Patricia J. Zondervan 1, Otto M. van Delden 3, Dirk J. Faber 2, Ton G. van Leeuwen 2, Jean J. de la Rosette 1, Daniel M. de Bruin 1,2, M. Pilar Laguna Pes 1
1Department of Urology, Academic Medical Center, 2Department of Biomedical Engineering and Physics, Academic Medical Center, 3Department of Radiology, Academic Medical Center

Optical coherence tomography (OCT) is a high resolution imaging technique that allows analysis of tissue specific optical properties providing the means for tissue differentiation. We developed needle based OCT, providing real-time imaging combined with on-the-spot tumor differentiation. This publication describes a method for percutaneous, needle based OCT of renal masses.

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Bioengineering

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
Sandra Van de Wiel 1, Maarten Merkx 2,3, Stan Van de Graaf 1
1Tytgat Institute for Liver and Intestinal Research, Department of Gastroenterology and Hepatology, Academic Medical Center, 2Laboratory of Chemical Biology, Institute of Complex Molecular Systems (ICMS), 3Department of Biomedical Engineering, Eindhoven University of Technology

We provide a detailed protocol to study bile acid dynamics in living cells using a genetically encoded BAS FRET sensor. This Bile Acid Sensor represents a unique tool to study (regulation of) bile acid transport and FXR activation in a wide range of cell types.

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Cancer Research

Laparoscopic Pancreatoduodenectomy With Modified Blumgart Pancreaticojejunostomy
Matteo De Pastena 1,2, Jony van Hilst 1, Thijs de Rooij 1, Olivier R Busch 1, Michael F Gerhards 3, Sebastiaan Festen 3, Marc G Besselink 1
1Department of Surgery, Cancer Center Amsterdam, Academic Medical Center, 2General and Pancreatic Surgery Department, Pancreas Institute, University and Hospital Trust of Verona, 3Department of Surgery, OLVG

Laparoscopic pancreatoduodenctomy (LPD) may offer advantages over open pancreatoduodenectomy, including early postoperative mobilization, less delayed gastric emptying and a shorter hospital stay. However, LPD is technically challenging and not well-standardized, especially regarding the pancreatic anastomosis. We describe a standardized technique for the pancreatic anastomosis during LPD: modified Blumgart pancreaticojejunostomy.

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