JoVE Logo

Sign In

Los Alamos National Laboratory

4 ARTICLES PUBLISHED IN JoVE

image

Biology

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
Miriam Cohen 1, Nissi M. Varki 1, Mark D. Jankowski 2, Pascal Gagneux 1
1Department of Cellular and Molecular Medicine, University of California, San Diego , 2Biosecurity and Public Health, Los Alamos National Laboratory

Unfixed frozen tissue samples embedded in Optimal Cutting Temperature medium (OCT) can be used to study natural distribution and glycosylation of secreted mucus. In this approach tissue processing is minimal and the natural presentation of glycolipids, mucins and glycan-epitopes is preserved. Tissue sections can be analyzed by immunohistochemistry using fluorescence or chromogenic detection.

image

Education

Ligand-Mediated Nucleation and Growth of Palladium Metal Nanoparticles
Saeed Mozaffari *1, Wenhui Li *1, Coogan Thompson 1, Sergei Ivanov 2, Soenke Seifert 3, Byeongdu Lee 4, Libor Kovarik 5, Ayman M. Karim 1
1Department of Chemical Engineering, Virginia Polytechnic Institute and State University, 2Center for Integrated Nanotechnologies, Los Alamos National Laboratory, 3Advanced Photon Source, Argonne National Laboratory, 4X-ray Science Division, Argonne National Laboratory, 5Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory

The main goal of this work is to elucidate the role of capping agents in regulating the size of palladium nanoparticles by combining in situ small angle x-ray scattering (SAXS) and ligand-based kinetic modeling.

image

Engineering

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments
Trevor Clark 1, Caitlin A. Taylor 1,2, Christopher M. Barr 1, Khalid Hattar 1
1Sandia National Laboratories, 2Los Alamos National Laboratory

Sample preparation techniques are outlined with specific considerations for in situ ion irradiation TEM experiments. Ion species, energy, and fluence are discussed with methods for how to select and compute them. Finally, procedures for conducting an experiment are described and accompanied by the representative results.

image

Medicine

Randomized Controlled Trial to Study the Acute Effects of Strength Exercise on Insulin Sensitivity in Obese Adults
Luis Filipe Rocha Silva 1, Bruna Caroline Chaves Garcia 2, Zach A. Mang 3, Fabiano Trigueiro Amorim 4, Marco Fabrício Dias-Peixoto 1, Fernando Gripp 1, Valmor Tricoli 5, Flávio de Castro Magalhães 1,4
1Department of Physical Education, Federal University of the Jequitinhonha and Mucuri Valleys, 2Laboratory of Exercise Biology and Immunometabolism, Centro Integrado de Pós-Graduação e Pesquisa em Saúde, Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, Federal University of the Jequitinhonha and Mucuri Valleys, 3Occupational Safety & Health, Los Alamos National Laboratory, 4Department of Health, Exercise, and Sports Sciences, University of New Mexico, 5School of Physical Education and Sport, University of Sao Paulo

This study describes a randomized controlled trial protocol aiming at assessing the acute effects of strength exercise volume on insulin sensitivity in obese individuals.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved