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Shinshu University School of Medicine

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

piggyBac Transposon System Modification of Primary Human T Cells
Sunandan Saha 1,2, Yozo Nakazawa 3, Leslie E. Huye 4,5, Joseph E. Doherty 2,6, Daniel L. Galvan 2, Cliona M. Rooney 4,5,7, Matthew H. Wilson 1,2,4,8
1Program in Translational Biology and Molecular Medicine, Baylor College of Medicine , 2Department of Medicine, Division of Nephrology, Baylor College of Medicine , 3Department of Immunology and Pathology, Shinshu University School of Medicine, 4Center for Cell and Gene Therapy, Baylor College of Medicine , 5Department of Pediatrics, Baylor College of Medicine , 6Program in Cell and Molecular Biology, Baylor College of Medicine , 7Department of Molecular Virology and Microbiology, Baylor College of Medicine , 8Michael E. DeBakey VA Medical Center

We describe a method to genetically modify primary human T cells with a transgene using the non-viral piggyBac transposon system. T cells modified to using the piggyBac transposon system exhibit stable transgene expression.

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Enhanced Cochlear Coverage and Hearing Preservation in High-Frequency Hearing Loss via Electric Acoustic Stimulation with Longer Electrode

Enhanced Cochlear Coverage and Hearing Preservation in High-Frequency Hearing Loss via Electric Acoustic Stimulation with Longer Electrode
Hidekane Yoshimura 1, Yutaka Takumi 1, Shin-ya Nishio 2, Shin-ichi Usami 2
1Department of Otorhinolaryngology - Head and Neck Surgery, Shinshu University School of Medicine, 2Department of Hearing Implant Sciences, Shinshu University School of Medicine

Electric acoustic stimulation (EAS) with longer electrodes can offer broader cochlear coverage and various types of maps in cases of high-frequency hearing loss. Combining less invasive surgery, flexible lateral-wall electrodes, and steroid administration permits deeper insertion with little or no surgical trauma, resulting in good preservation of hearing.

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