JoVE Logo
Faculty Resource Center

Sign In

piggyBac Transposon System Modification of Primary Human T Cells

DOI :

10.3791/4235-v

November 5th, 2012

November 5th, 2012

16,768 Views

1Program in Translational Biology and Molecular Medicine, Baylor College of Medicine , 2Department of Medicine, Division of Nephrology, Baylor College of Medicine , 3Department of Immunology and Pathology, Shinshu University School of Medicine, 4Center for Cell and Gene Therapy, Baylor College of Medicine , 5Department of Pediatrics, Baylor College of Medicine , 6Program in Cell and Molecular Biology, Baylor College of Medicine , 7Department of Molecular Virology and Microbiology, Baylor College of Medicine , 8Michael E. DeBakey VA Medical Center

We describe a method to genetically modify primary human T cells with a transgene using the non-viral piggyBac transposon system. T cells modified to using the piggyBac transposon system exhibit stable transgene expression.

-- Views

Related Videos

article

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

article

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System. (Video) | JoVE

article

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis (Video) | JoVE

article

Generation of Human Alloantigen-specific T Cells from Peripheral Blood (Video) | JoVE

article

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs) (Video) | JoVE

article

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (ITregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol (Video) | JoVE

article

Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells (Video) | JoVE

article

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation (Video) | JoVE

article

High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection (Video) | JoVE

article

Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved