University of North Carolina at Greensboro

3 ARTICLES PUBLISHED IN JoVE

Chemistry

Synthesis of Hypervalent Iodonium Alkynyl Triflates for the Application of Generating Cyanocarbenes

I. F. Dempsey Hyatt¹, Daniel J. Nasrallah¹, Mitchell P. Croatt¹

¹Department of Chemistry and Biochemistry, University of North Carolina at Greensboro

Herein is described the synthesis, isolation, and reactions of hypervalent iodonium alkynyl triflates (HIATs) with azides to generate cyanocarbenes. The procedures will involve air-sensitive and cryogen techniques, including cold filtration under an inert atmosphere. Handling and safety of the synthesized compounds will also be discussed.

Biology

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

Stephen Vance^*1, Effat Zeidan^*1, Vincent C. Henrich², Marinella G. Sandros³

¹Department of Nanoscience, University of North Carolina at Greensboro, ²Center for Biotechnology, Genomics, and Health Research, University of North Carolina at Greensboro, ³HORIBA Scientific

The proposed work assesses the diagnostic potential of direct and amplified surface plasmon resonance imaging (SPRi) assays, particularly for the detection of recombinant human growth hormone in spiked human serum, by comparing SPRi results directly with commercially available enzyme-linked immunosorbent assay (ELISA) kit.

Chemistry

A Study of the Complexation of Mercury(II) with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry

Johanna Mazlo¹, Maria Ngu-Schwemlein²

¹Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, ²Department of Chemistry, Winston-Salem State University

The characterization of complexes formed in different relative ratios of mercury(II) to dicysteinyl tetrapeptides by electrospray ionization orbitrap mass spectrometry is presented.