Virginia Tech Carilion Research Institute

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

In situ TEM of Biological Assemblies in Liquid

Madeline J. Dukes^*1, Brian L. Gilmore^*2, Justin R. Tanner^*2, Sarah M. McDonald², Deborah F. Kelly²

¹Applications Science, Protochips, Inc., ²Virginia Tech Carilion Research Institute

Here we describe a procedure to image viral complexes in liquid at nanometer resolution using a transmission electron microscope.

Neuroscience

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Konark Mukherjee¹, Helen R. Clark¹, Vrushali Chavan¹, Emily K. Benson², Grahame J. Kidd², Sarika Srivastava¹

¹Virginia Tech Carilion Research Institute, ²Renovo Neural Incorporated

Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.

Biochemistry

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Liza-Anastasia DiCecco^*1,2, Samantha Berry^*1, G. M. Jonaid^*1,3, Maria J. Solares^1,4, Liam Kaylor^1,4, Jennifer L. Gray⁵, Carol Bator⁶, William J. Dearnaley¹, Michael Spilman⁷, Madeline J. Dressel-Dukes⁸, Kathryn Grandfield², Sarah M. McDonald Esstman⁹, Deborah F. Kelly^1,5,6

¹Department of Biomedical Engineering, Pennsylvania State University, ²Department of Materials Science and Engineering, McMaster University, ³Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, ⁴Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, ⁵Materials Research Institute, Pennsylvania State University, ⁶Huck Institutes of the Life Sciences, Pennsylvania State University, ⁷Applications team, Direct Electron, ⁸Application Scientist, Protochips, Inc., ⁹Department of Biology, Wake Forest University

Here protocols are described to prepare virus assemblies suitable for liquid-EM and cryo-EM analysis at the nanoscale using transmission electron microscopy.