An erratum was issued for: In situ TEM of Biological Assemblies in Liquid. The Representative Results and References sections were updated.
Here we describe a procedure to image viral complexes in liquid at nanometer resolution using a transmission electron microscope.
Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.
Here protocols are described to prepare virus assemblies suitable for liquid-EM and cryo-EM analysis at the nanoscale using transmission electron microscopy.