Accedi

Virginia Tech Carilion Research Institute

4 ARTICLES PUBLISHED IN JoVE

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Bioengineering

In situ TEM of Biological Assemblies in Liquid
Madeline J. Dukes *1, Brian L. Gilmore *2, Justin R. Tanner *2, Sarah M. McDonald 2, Deborah F. Kelly 2
1Applications Science, Protochips, Inc., 2Virginia Tech Carilion Research Institute

Here we describe a procedure to image viral complexes in liquid at nanometer resolution using a transmission electron microscope.

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Neuroscience

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy
Konark Mukherjee 1, Helen R. Clark 1, Vrushali Chavan 1, Emily K. Benson 2, Grahame J. Kidd 2, Sarika Srivastava 1
1Virginia Tech Carilion Research Institute, 2Renovo Neural Incorporated

Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.

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Biochemistry

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
Liza-Anastasia DiCecco *1,2, Samantha Berry *1, G. M. Jonaid *1,3, Maria J. Solares 1,4, Liam Kaylor 1,4, Jennifer L. Gray 5, Carol Bator 6, William J. Dearnaley 1, Michael Spilman 7, Madeline J. Dressel-Dukes 8, Kathryn Grandfield 2, Sarah M. McDonald Esstman 9, Deborah F. Kelly 1,5,6
1Department of Biomedical Engineering, Pennsylvania State University, 2Department of Materials Science and Engineering, McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 5Materials Research Institute, Pennsylvania State University, 6Huck Institutes of the Life Sciences, Pennsylvania State University, 7Applications team, Direct Electron, 8Application Scientist, Protochips, Inc., 9Department of Biology, Wake Forest University

Here protocols are described to prepare virus assemblies suitable for liquid-EM and cryo-EM analysis at the nanoscale using transmission electron microscopy.

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