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Chungnam National University

6 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Automated Gait Analysis in Mice with Chronic Constriction Injury
Dong-Wook Kang 1, Jae-Gyun Choi 1, Ji-Young Moon 2, Suk-Yun Kang 2, Yeonhee Ryu 2, Jin Bong Park 1, Hyun-Woo Kim 1,3
1Department of Physiology and Medical Science, College of Medicine and Brain Research Institute, Chungnam National University, 2KM Fundamental Research Division, Korea Institute of Oriental Medicine (KIOM), 3Department of Neuroscience and Cell Biology, University of Texas Medical Branch at Galveston

The precise assessment of pain response in a neuropathic animal model is critical to investigate the pathophysiology of pain diseases and develop new analgesics. We present a sensitive and objective method to determine the sensory function of the rodent hind paw by an automated gait analysis system.

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Genetics

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
Seoghyun Lee *1, Minho Won *2, Ran Hee Hwang 3, Gang Min Hur 2, Hyunju Ro 1
1Department of Biological Sciences, College of Bioscience and Biotechnology, Chungnam National University, 2Department of Pharmacology, College of Medicine, Chungnam National University, 3Department of Nursing, Gwangju Women's University

Here, we present a protocol for the modulation of transgene expression using pEUI(+) singular gene switch by tebufenozide treatment.

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Immunology and Infection

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation
Le Hong Son *1, Liu Ye *1, Inkyu Hwang 1
1College of Pharmacy, Chungnam National University

We have established an ex vivo mast cell degranulation assay carried out by incubating crude peritoneal exudate cells isolated from the mice, treated with a pharmacological agent of interest and administered anti-dinitrophenol (DNP) IgE beforehand, with DNP on a carrier protein.

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Neuroscience

Burn Injury-Induced Pain and Depression-Like Behavior in Mice
Jae-Gyun Choi *1, Dong-Wook Kang *1, Jaehyuk Kim 1, Miae Lee 1, Sheu-Ran Choi 2, Jin Bong Park 1, Hyun-Woo Kim 1
1Department of Physiology and Medical Science, College of Medicine and Brain Research Institute, Chungnam National University, 2Department of Pharmacology, Catholic Kwandong University College of Medicine

A transient scald injury (65 °C ± 0.5 °C, 3 s) of one hind paw decreases the threshold (g) to von Frey filament stimulation of the ipsilateral side and alters gait pattern. Besides, burn injury induces depression-like behavior in the forced swimming test.

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Neuroscience

In Vivo Whole-Brain Imaging of Zebrafish Larvae Using Three-Dimensional Fluorescence Microscopy
Eun-Seo Cho 1, Seungjae Han 1, Gyuri Kim 1, Minho Eom 1, Kang-Han Lee 2, Cheol-Hee Kim 2, Young-Gyu Yoon 1,3
1School of Electrical Engineering, KAIST, 2Department of Biology, Chungnam National University, 3KAIST Institute for Health Science and Technology

Presented here is a protocol for in vivo whole-brain imaging of larval zebrafish using three-dimensional fluorescence microscopy. The experimental procedure includes sample preparation, image acquisition, and visualization.

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Biochemistry

Arteriovenous Metabolomics to Measure In Vivo Metabolite Exchange in Brown Adipose Tissue
Sanghun Lee *1, Gyumin Lim *2,3, Soyeon Kim *1, Hyoju Kim 1, Yeon Jin Roh 3, Wantae Kim 2, Dong Wook Choi 3, Su Myung Jung 1
1Department of Biological Sciences, Sungkyunkwan University (SKKU), 2Department of Biochemistry, College of Natural Sciences, Chungnam National University, 3Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University

In this protocol, methods relevant for BAT-optimized arteriovenous metabolomics using GC-MS in a mouse model are outlined. These methods allow for the acquisition of valuable insights into BAT-mediated metabolite exchange at the organismal level.

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