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Guizhou Medical University

5 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats
Aoshuang Chang 1,2, Cuixian Li 1, Jianfeng Huang 1,3, Wenlu Pan 1, Yinghong Tian 4, Jie Tang 1
1Department of Physiology, School of Basic Medical Sciences, Southern Medical University, 2School of Basic Medical Sciences, Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region, Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences, Southern Medical University

This protocol describes methods to record the auditory brainstem response from postnatal rat pups. To examine the functional development of outer hair cells, the experimental procedure of whole-cell patch clamp recording in isolated outer hair cells is described step-by-step.

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Immunology and Infection

Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines
Lingling Chen *1,2, Qiuqiu Xiao *1, Mengting Shi 1, Jinzhi Cheng 1, Jiahong Wu 1
1Guizhou Medical University, 2Guizhou Health Vocational College

Four methods were used to detect intracellular Wolbachia, which complemented each other and improved the detection accuracy of Wolbachia infection of Aedes albopictus-derived Aa23 and Aa23-T cured of native Wolbachia infection using antibiotics.

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Biology

Control of Aedes albopictus Mosquito Larvae with Carpesium abrotanoides L.
Qiuguo Liang 1,2, Xi Yang 1,2, Dejun Liao 1,2, Cheng Yi 1,2, Rongmin Dang 1,2, Jiahong Wu 3, Lin Huang 1,2
1Qiannan Medical College for Nationalities, 2Key Laboratory of Human Parasitic Diseases in Qiannan Prefecture, 3Guizhou Medical University

Using the Carpesium abrotanoides L. plant for mosquito larvae control can effectively reduce the Aedes albopictus mosquito population and provide a foundation for designing plant-derived insecticides.

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Cancer Research

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer
Dingwen Xu *1, Xiaofei Hu *2, Yang Feng 1, Zhihua Lu 1, Xianmei Liu 3, Huishen Yan 1, Jianming Peng 1, Sen Luo 1, Weijuan Yao 4
1Department of Clinic, School of Medicine, Yangzhou Polytechnic College, 2Department of Nuclear Medicine, Huangshi Central Hospital, Affiliated Hospital of Hubei Institute of Technology, 3School of Biology and Engineering, Guizhou Medical University, 4Hemorheology Center, Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center

Tropomodulin 3 (TMOD3) has been increasingly studied in tumors in recent years. This study is the first to report that TMOD3 is highly expressed in ovarian cancer and is closely associated with platinum resistance and immune infiltration. These results could help improve the therapeutic outcomes for ovarian cancer.

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Biology

Generating the Transcriptional Regulation View of Transcriptomic Features for Prediction Task and Dark Biomarker Detection on Small Datasets
Kewei Li 1, Yusi Fan 1, Yaqing Liu 1, Hongmei Liu 2, Gongyou Zhang 2, Meiyu Duan 1, Lan Huang 1, Fengfeng Zhou 1
1College of Computer Science and Technology, and Key Laboratory of Symbolic Computation and Knowledge Engineering of Ministry of Education, Jilin University, 2School of Biology and Engineering, Guizhou Medical University

Here, we introduce a protocol for converting transcriptomic data into a mqTrans view, enabling the identification of dark biomarkers. While not differentially expressed in conventional transcriptomic analyses, these biomarkers exhibit differential expression in the mqTrans view. The approach serves as a complementary technique to traditional methods, unveiling previously overlooked biomarkers.

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