yeditepe university

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions

Ayşegül Doğan¹, Selami Demirci², Hüseyin Apdik¹, Ezgi Avşar Apdik¹, Fikrettin Şahin¹

¹Genetics and Bioengineering, Yeditepe University, ²National Heart, Lung, and Blood Institute (NHLBI), Sickle Cell Branch, National Institutes of Health (NIH)

We provide protocols for evaluation of mesenchymal stem cells isolated from dental pulp and prostate cancer cell interactions based on direct and indirect co-culture methods. Condition medium and trans-well membranes are suitable to analyze indirect paracrine activity. Seeding differentially stained cells together is an appropriate model for direct cell-cell interaction.

Medicine

Impact of Fabrication Techniques and Polishing Procedures on Surface Roughness of Denture Base Resins

Yunus Emre Ozden¹, Pinar Yilmaz Atali², Zeynep Ozkurt Kayahan¹

¹Faculty of Dentistry Department of Prosthodontics, Yeditepe University, ²Faculty of Dentistry Department of Restorative Dentistry, Marmara University

This article presents a protocol to evaluate the effects of different manufacturing methods (heat-polymerized PMMA, CAD-CAM milled PMMA, and 3D-printed resin) and polishing techniques (600, 800, and 1000 grit silicon carbide abrasive papers) on the surface roughness (Ra) of resin base materials used for complete dentures.