Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their InteractionsAyşegül Doğan 1, Selami Demirci 2, Hüseyin Apdik 1, Ezgi Avşar Apdik 1, Fikrettin Şahin 1
1Genetics and Bioengineering, Yeditepe University, 2National Heart, Lung, and Blood Institute (NHLBI), Sickle Cell Branch, National Institutes of Health (NIH)
We provide protocols for evaluation of mesenchymal stem cells isolated from dental pulp and prostate cancer cell interactions based on direct and indirect co-culture methods. Condition medium and trans-well membranes are suitable to analyze indirect paracrine activity. Seeding differentially stained cells together is an appropriate model for direct cell-cell interaction.
