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yamaguchi university

3 ARTICLES PUBLISHED IN JoVE

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Behavior

A Common Marmoset Model of Mother-Infant Intervention for Breastfeeding Disorders in the Presence of Paternal Inhibition and Maternal Neglect
Mayuko Yoda 1,2, Yoshimasa Kamei 3, Hayato Sakurai 4, Hiroko Kakei 4, Ting Tao 1,2,4, Hideo Yamanouchi 4, Tetsuya Kunikata 4, Masanori Hariyama 5, Ricki Colman 6, Mamiko Koshiba 1,4,5
1Graduate School of Sciences and Technology for Innovation, Yamaguchi University, 2Faculty of Health Sciences and Nursing, Juntendo University, 3Obstetrician, Medical Department, Saitama Medical University, 4Department of Pediatrics, Saitama Medical University, 5Graduate School of Information Sciences, Tohoku University, 6Department of Cell and Regenerative Biology and Wisconsin National Primate Research Center, University of Wisconsin, Madison

Here, we present a nonhuman primate model of mother-infant intervention for breastfeeding disorders in the presence of paternal inhibition and maternal neglect. The mate model video complements education to support primate and human caregivers with respect to infants with breastfeeding problems such as pain.

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Behavior

Four Temporary Waterslide Designs Adapted to Different Slope Conditions to Encourage Child Socialization in Playgrounds
Zhudi Hua 1, Ting Tao 1, Risa Akita 1, Tomofusa Akita 1, Yoshiaki Hayakawa 1, Masanori Hariyama 2, Hayato Sakurai 3, Ricki Colman 4, Mamiko Koshiba 1,2,3
1Graduate School of Sciences and Technology for Innovation, Yamaguchi University, 2Graduate School of Information Sciences, Tohoku University, 3Pediatrics, Saitama Medical University Hospital, 4Department of Cell and Regenerative Biology, University of Wisconsin-Madison

Early life social learning is enhanced by interactions with effectively designed environments. Four events were held at different city parks using inexpensive, temporary waterslides to stimulate social learning. This study describes the prototypes used and the evaluation of the children's interactions.

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Bioengineering

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype
Kinya Matsuo 1, Britta Engelhardt 2, Hideaki Nishihara 3
1Graduate School of Medicine, Yamaguchi University, 2Theodor Kocher Institute, University of Bern, 3Department of Neurotherapeutics, Yamaguchi University of Medicine

Here, we describe a protocol, the extended endothelial cell culture method (EECM), that allows differentiation of pluripotent stem cells to brain microvascular endothelial cell (BMEC)-like cells. These cells show endothelial cell adhesion molecule expression and are thus a human blood-brain barrier model suitable to study immune cell interactions in vitro.

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