National Defense Medical College

3 ARTICLES PUBLISHED IN JoVE

Medicine

Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches

Kazuhiko Shirakabe^1,2, Masaaki Higashiyama¹, Naoki Shibuya², Kazuki Horiuchi¹, Masayuki Saruta², Ryota Hokari¹

¹Department of internal medicine, National Defense Medical College, ²Division of Gastroenterology and Hepatology, Department of Internal Medicine, Jikei University School of Medicine

Here, we describe a precise method for collecting thoracic duct lymphocytes and observing the migration of gut-tropic lymphocytes in rat Peyer’s patches for 3 hours using time-lapse photography. This technique can clarify how the dynamics of lymphocytes are affected under inflammatory conditions.

Medicine

Research Application of Laser-Induced Shock Wave for Studying Blast-Induced Cochlear Injury

Takaomi Kurioka¹, Kunio Mizutari¹, Katsuki Niwa¹, Eiko Kimura¹, Satoko Kawauchi², Yasushi Kobayashi³, Shunichi Sato²

¹Department of Otolaryngology, Head and Neck Surgery, National Defense Medical College, ²Division of Bioinformation and Therapeutic Systems, National Defense Medical College, ³Department of Biochemistry, National Defense Medical College

Here, we describe experimental protocols for creating an animal model of blast-induced cochlear injury using laser-induced shock wave (LISW). Exposure of the temporal bone to LISW allows the reproduction of blast-induced cochlear pathophysiology. This animal model could be a platform for elucidating cochlear pathology and exploring potential treatments for blast injuries.

Neuroscience

Evaluation of Blood-Brain Barrier Breakdown in a Mouse Model of Mild Traumatic Brain Injury

Kiyomasa Nishii¹, Takahito Higashi¹, Toshiaki Ishizuka², Yasushi Satoh³, Yasushi Kobayashi¹

¹Department of Anatomy and Neurobiology, National Defense Medical College, ²Department of Pharmacology, National Defense Medical College, ³Department of Biochemistry, National Defense Medical College

A method was developed to visualize dye extravasation due to blood-brain barrier (BBB) breakdown by administering two fluorescent dyes to mice at different time points. The use of glycerol as a cryoprotectant facilitated immunohistochemistry on the same sample.