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Ludwig-Maximilians University of Munich

4 ARTICLES PUBLISHED IN JoVE

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Biology

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)
Samira Samtleben 1, Juliane Jaepel 1,2, Caroline Fecher 1, Thomas Andreska 1, Markus Rehberg 3, Robert Blum 1
1Institute for Clinical Neurobiology, University of Wuerzburg, 2Department of Synapses - Circuits - Plasticity, Max Planck Institute of Neurobiology, Martinsried, 3Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians University of Munich

Targeted-esterase induced dye loading (TED) supports the analysis of intracellular calcium store dynamics by fluorescence imaging. The method bases on targeting of a recombinant Carboxylesterase to the endoplasmic reticulum (ER), where it improves the local unmasking of synthetic low-affinity Ca2+ indicator dyes in the ER lumen.

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Biochemistry

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
Louisa Jutta Dietz 1, Anuroop Venkateswaran Venkatasubramani 1, Annika Müller-Eigner 2, Martin Hrabe de Angelis 3,4,5, Axel Imhof 1, Lore Becker 3, Shahaf Peleg 2,6,7
1Munich Center of Integrated Protein Science and Biomedical Center, Ludwig-Maximilians University of Munich, 2Laboratory for Metabolism and Epigenetics in Aging, Leibniz Institute for Farm Animal Biology (FBN), 3German Mouse Clinic, Helmholtz Zentrum Munich, German Research Center for Environment and Health (GmbH), 4German Center for Diabetes Research (DZD), 5Chair of Experimental Genetics, School of Life Science Weihenstephan, Technische Universität München, 6Laboratory for Metabolism and Epigenetics in Brain Aging, Institute of Neuroregeneration & Neurorehabilitation of Qingdao University, 7Molecular Biology Division, Biomedical Center, Faculty of Medicine, Ludwig-Maximilians University of Munich

Measuring alterations in metabolic rates is central to understanding the progression of various diseases and aging. Here, we present a novel technique to measure whole head oxygen consumption that more closely resembles the physiological state and may aid in revealing novel drugs that modify mitochondrial activity.

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Genetics

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins
Isabell-Christin Fiedler 1, Arne Weiberg 2, Karina van der Linde 1
1Department of Biology, Regensburg University, 2Department of Biology, Ludwig-Maximilians University of Munich

This work describes the cloning of an Ustilago maydis Trojan horse strain for the in situ delivery of secreted maize proteins into three different types of maize tissues.

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Immunology and Infection

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
Qin Xiao *1, Yuheng Liu *1, Dandan Zhang 1, Chao Li 1, Qihua Yang 1, Dongshui Lu 1, Weijun Zhang 1, Joseph Rosenecker 2, Quanming Zou 1, Yang Li 3, Shan Guan 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University

A self-assembled peptide-poloxamine nanoparticle (PP-sNp) is developed using a microfluidic mixing device to encapsulate and deliver in vitro transcribed messenger RNA. The described mRNA/PP-sNp could efficiently transfect cultured cells in vitro.

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