Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
15.6K Views
•
07:11 min
•
January 7th, 2019
DOI :
January 7th, 2019
•Louisa Jutta Dietz1, Anuroop Venkateswaran Venkatasubramani1, Annika Müller-Eigner2, Martin Hrabe de Angelis3,4,5, Axel Imhof1, Lore Becker3, Shahaf Peleg2,6,7
1Munich Center of Integrated Protein Science and Biomedical Center, Ludwig-Maximilians University of Munich, 2Laboratory for Metabolism and Epigenetics in Aging, Leibniz Institute for Farm Animal Biology (FBN), 3German Mouse Clinic, Helmholtz Zentrum Munich, German Research Center for Environment and Health (GmbH), 4German Center for Diabetes Research (DZD), 5Chair of Experimental Genetics, School of Life Science Weihenstephan, Technische Universität München, 6Laboratory for Metabolism and Epigenetics in Brain Aging, Institute of Neuroregeneration & Neurorehabilitation of Qingdao University, 7Molecular Biology Division, Biomedical Center, Faculty of Medicine, Ludwig-Maximilians University of Munich
Chapters in this video
0:04
Title
0:33
Instrument Preparation
1:24
Cartridge Preparation
2:21
Plate Preparation
4:37
Analysis of OCR Measurements
5:12
Results: Oxygen Levels of Midlife Heads Lower than Young Heads
6:42
Conclusion
Related Videos
10:49
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
12.5K Views
08:56
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
10.8K Views
14:55
Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue
11.8K Views
07:12
A Colorimetric Method for Measuring Iron Content in Plants
21.9K Views
07:57
Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
13.8K Views
An Affordable and Efficient "Homemade" Platform for Drosophila Behavioral Studies, and an Accompanying Protocol for Larval Mitochondrial Respirometry
841 Views
07:06
Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains
9.2K Views
04:31
Measurement of Metabolic Rate in Drosophila using Respirometry
19.5K Views
07:12
Measuring O2 Consumption in Drosophila melanogaster Using Coulometric Microrespirometry
891 Views
08:10
Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans
8.9K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved