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University Hospitals Leuven

10 ARTICLES PUBLISHED IN JoVE

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Medicine

A Novel Surgical Approach for Intratracheal Administration of Bioactive Agents in a Fetal Mouse Model
Marianne S. Carlon *1, Jaan Toelen *2, Marina Mori da Cunha 2, Dragana Vidović 1, Anke Van der Perren 3, Steffi Mayer 2, Lourenço Sbragia 2, Johan Nuyts 4, Uwe Himmelreich 5, Zeger Debyser 1, Jan Deprest 2
1Molecular Virology and Gene Therapy, KU Leuven, 2Department of Woman and Child, KU Leuven, 3Neurobiology and Gene Therapy, KU Leuven, 4Division of Nuclear Medicine, KU Leuven, 5Biomedical NMR Unit/ MoSAIC, KU Leuven

We developed a novel surgical approach for intratracheal administration of bioactive agents into the mouse fetus. The delivery route is more efficient in targeting the fetal mouse lungs than the commonly used intra-amniotic injection. This procedure has to date not been described in a mouse model.

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Medicine

Fetal Echocardiography and Pulsed-wave Doppler Ultrasound in a Rabbit Model of Intrauterine Growth Restriction
Ryan Hodges 1,2, Masayuki Endo 1, Andre La Gerche 3, Elisenda Eixarch 4,5, Philip DeKoninck 1, Vessilina Ferferieva 3, Jan D'hooge 3, Euan M. Wallace 2, Jan Deprest 1
1Division Woman and Child, Department Women, University Hospitals Leuven, 2The Ritchie Centre, Monash Institute of Medical Research, Department of Obstetrics and Gynaecology, Monash University, Victoria, Australia, 3Department of Cardiovascular Sciences, Katholieke Universiteit Leuven, 4Fetal and Perinatal Medicine Research Group, Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), 5Maternal-Fetal Medicine Department, ICGON, Hospital Clínic, Universitat de Barcelona, Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER)

We describe examination of fetal cardiac function with contemporary functional fetal echocardiography and fetoplacental Doppler ultrasound using the VisualSonics VEVO 2100 microultrasound in a surgically induced model of intrauterine fetal growth restriction in a rabbit.

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Biology

Rapid Analysis and Exploration of Fluorescence Microscopy Images
Benjamin Pavie *1, Satwik Rajaram *1, Austin Ouyang 2, Jason M. Altschuler 1,3, Robert J. Steininger III 1, Lani F. Wu 1, Steven J. Altschuler 1
1Green Center for Systems Biology, UT Southwestern Medical Center, 2Advanced Imaging Research Center, UT Southwestern Medical Center, 3Princeton University

Here we describe a workflow for rapidly analyzing and exploring collections of fluorescence microscopy images using PhenoRipper, a recently developed image-analysis platform.

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Bioengineering

Medical-grade Sterilizable Target for Fluid-immersed Fetoscope Optical Distortion Calibration
Daniil I. Nikitichev *1, Dzhoshkun I. Shakir *1, François Chadebecq 1, Marcel Tella 1, Jan Deprest 1,2, Danail Stoyanov 3, Sébastien Ourselin 1, Tom Vercauteren 1
1Translational Imaging Group, CMIC, University College London, 2Department of Obstetrics and Gynecology, University Hospitals Leuven, 3Surgical Robot Vision Group, CMIC, University College London

This article describes the design and development of a sterilizable custom camera optical distortion calibration target for the peri-operative, fluid-immersed calibration of endoscopes during endoscopic interventions.

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Medicine

Transvaginal Mesh Insertion in the Ovine Model
Iva Urbankova 1,2, Geertje Callewaert 1,3, Nikhil Sindhwani 1, Alice Turri 1, Lucie Hympanova 1,2, Andrew Feola 1, Jan Deprest 1,3
1Centrum for Surgical Technologies, Department of Development and Regeneration, Clinical Specialties Research Groups, Faculty of Medicine, KU Leuven, 2Institute for the Care of Mother and Child and Third Faculty of Medicine, Charles University, Prague, 3Pelvic Floor Unit, University Hospitals KU Leuven

This protocol describes mesh implantation in the ovine rectovaginal septum using a single vaginal incision technique, with and without the trocar-guided insertion of anchoring arms.

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Neuroscience

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration
Eveliina Pollari 1,2, Robert Prior 1,2, Wim Robberecht 1,2,3, Philip Van Damme 1,2,3, Ludo Van Den Bosch 1,2
1Department of Neurosciences, Experimental Neurology, KU Leuven - University of Leuven, 2Center for Brain & Disease Research, Laboratory of Neurobiology, VIB, 3Department of Neurology, University Hospitals Leuven

The measurement of nerve conduction is a useful tool to assess mouse models of neurodegeneration but it is frequently only applied to stimulate the sciatic nerve in hindlimbs. Here, we describe a technique to measure compound muscle action potential (CMAP) in vivo in the mouse forelimb muscles innervated by the brachial plexus.

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Chemistry

Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery
Lei Nie 1,2, Shaolan Sun 1, Meng Sun 1, Qiuju Zhou 3, Zongwen Zhang 3, Lingyun Zheng 3, Lingling Wang 3
1College of Life Sciences, Xinyang Normal University, 2Department of Imaging & Pathology, University of Leuven and Oral & Maxillofacial Surgery, University Hospitals Leuven, 3Analysis & Testing Center, Xinyang Normal University

In this protocol, doxorubicin-loaded AS1411-g-PEI-g-PEG modified gold nanoparticles are synthesized via three-step amide reactions. Then, doxorubicin is loaded and delivered to target cancer cells for cancer therapy.

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Neuroscience

Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices
Katarina Stoklund Dittlau 1,2, Emily N. Krasnow 1,2, Laura Fumagalli 1,2, Tijs Vandoorne 1,2, Pieter Baatsen 3,4, Axelle Kerstens 3,4, Giorgia Giacomazzi 5, Benjamin Pavie 3,4, Elisabeth Rossaert 1,2, Jimmy Beckers 1,2, Maurilio Sampaolesi 5, Philip Van Damme 1,2,6, Ludo Van Den Bosch 1,2
1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute, KU Leuven - University of Leuven, 2VIB Bio Imaging Core, KU Leuven - University of Leuven, 3Department of Development and Regeneration, Stem Cell and Developmental Biology, KU Leuven - University of Leuven, 4Department of Neurology, University Hospitals Leuven

We describe a method to generate human motor units in commercially available microfluidic devices by co-culturing human induced pluripotent stem cell-derived motor neurons with human primary mesoangioblast-derived myotubes resulting in the formation of functionally active neuromuscular junctions.

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Medicine

Rectal Organoid Morphology Analysis (ROMA): A Diagnostic Assay in Cystic Fibrosis
Senne Cuyx 1,2, Anabela S. Ramalho 1, Nikky Corthout 3,4, Steffen Fieuws 5, Eva Fürstová 6, Kaline Arnauts 7,8, Marc Ferrante 7,9, Catherine Verfaillie 8, Sebastian Munck 3,4, Mieke Boon 1,2, Marijke Proesmans 1,2, Lieven Dupont 10,11, Kris De Boeck 1,2, François Vermeulen 1,2
1Department of Development and Regeneration, Woman and Child Unit, CF research lab, KU Leuven, 2Department of Pediatrics, Pediatric Pulmonology, University Hospitals Leuven, 3VIB Bio Imaging Core, VIB-KU Leuven Center for Brain & Disease Research, 4Department for Neuroscience, KU Leuven, 5Interuniversity Center for Biostatistics and Statistical Bioinformatics, University of Leuven and University of Hasselt, 6Department of Pediatrics, 2nd Faculty of Medicine, Charles University and Motol University Hospital, 7Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, 8Department of Development and Regeneration, Stem Cell Institute Leuven (SCIL), KU Leuven, 9Department of Gastroenterology and Hepatology, University Hospitals Leuven, KU Leuven, 10Department of Chronic Diseases, Metabolism and Ageing; Pneumology, KU Leuven, 11Department of Respiratory Diseases, University Hospitals Leuven

This protocol describes rectal organoid morphology analysis (ROMA), a novel diagnostic assay for cystic fibrosis (CF). Morphological characteristics, namely the roundness (circularity index, CI) and the presence of a lumen (intensity ratio, IR), are a measure of CFTR function. Analysis of 189 subjects showed perfect discrimination between CF and non-CF.

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Medicine

Porcine Normothermic Isolated Liver Perfusion
Joris Blondeel 1,2, Nicholas Gilbo 1,2, Tine Wylin 1, Veerle Heedfeld 1, Diethard Monbaliu 1,2
1Department of Microbiology, Immunology and Transplantation, Laboratory of Abdominal Transplantation, KU Leuven, 2Department of Abdominal Transplant Surgery and Coordination, University Hospitals Leuven

The porcine model of liver normothermic machine perfusion (NMP), described here, can be successfully used to study NMP as a preservation strategy, a tool for viability assessment, and a platform for organ repair. It holds a high translational value, however it is technically challenging and labor-intensive.

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