Rectal Organoid Morphology Analysis (ROMA): A Diagnostic Assay in Cystic Fibrosis

2.7K Views

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07:56 min

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June 10th, 2022

DOI :

10.3791/63818-v

June 10th, 2022

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Senne Cuyx¹^,², Anabela S. Ramalho¹, Nikky Corthout³^,⁴, Steffen Fieuws⁵, Eva Fürstová⁶, Kaline Arnauts⁷^,⁸, Marc Ferrante⁷^,⁹, Catherine Verfaillie⁸, Sebastian Munck³^,⁴, Mieke Boon¹^,², Marijke Proesmans¹^,², Lieven Dupont¹⁰^,¹¹, Kris De Boeck¹^,², François Vermeulen¹^,²

¹Department of Development and Regeneration, Woman and Child Unit, CF research lab, KU Leuven, ²Department of Pediatrics, Pediatric Pulmonology, University Hospitals Leuven, ³VIB Bio Imaging Core, VIB-KU Leuven Center for Brain & Disease Research, ⁴Department for Neuroscience, KU Leuven, ⁵Interuniversity Center for Biostatistics and Statistical Bioinformatics, University of Leuven and University of Hasselt, ⁶Department of Pediatrics, 2<sup>nd</sup> Faculty of Medicine, Charles University and Motol University Hospital, ⁷Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, ⁸Department of Development and Regeneration, Stem Cell Institute Leuven (SCIL), KU Leuven, ⁹Department of Gastroenterology and Hepatology, University Hospitals Leuven, KU Leuven, ¹⁰Department of Chronic Diseases, Metabolism and Ageing; Pneumology, KU Leuven, ¹¹Department of Respiratory Diseases, University Hospitals Leuven

Summary

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Rectal Organoid Morphology Analysis

Cystic Fibrosis

Organoid Analysis

Calcium Green Staining

Live Cell Imaging

Confocal Microscope

Image Analysis Software

Morphological Differences

Organoid Culture

Fluorescence Distribution

Image Recalibration

Chapters in this video

0:04

Introduction

0:59

Organoid Imaging Using Confocal Microscopy

2:49

Image Analysis and Measuring the Indices in the Imaging Software

5:55

Results: Morphological Characteristics Determination Using Rectal Organoid Morphology Analysis

7:13

Conclusion

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