Kansai Medical University

3 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Preparing Activated Platelet-Rich Plasma for Culturing Human Adipose-Derived Stem Cells

Fangyuan Lai^1,2, Natsuko Kakudo¹, Naoki Morimoto¹, Yuanyuan Ma¹, Kenji Kusumoto¹

¹Department of Plastic and Reconstructive Surgery, Kansai Medical University, ²Department of Plastic Surgery, Zhejiang Provincial People's Hospital

In this report, we describe a double-spin method for preparing activated platelet-rich plasma (PRP). Using autologous thrombin, human adipose-derived stem cells (hASCs) were cultured. Activated PRP was shown to promote proliferation of hASCs.

Bioengineering

A Novel Cell Injection Method with Minimum Invasion

Fumiyuki Hattori¹

¹Innovative Regenerative Medicine, Graduate School of Medicine, Kansai Medical University

This method eliminates any major invasion during cell injections caused by the cell suspension solution.

Biology

Application of Live Mitochondria Staining in Cell-Sorting to Purify Hepatocytes Derived from Human Induced Pluripotent Stem Cells

Hiromi Yamashita¹, Fumiyuki Hattori¹

¹Innovative Regenerative Medicine, Graduate School of Medicine, Kansai Medical University

Hepatocytes derived from pluripotent stem cells can be purified through cell sorting, using a combination of mitochondrial and activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) staining.