BC Children's Hospital

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis

Ganive Bhinder¹, Ho Pan Sham¹, Justin M. Chan¹, Vijay Morampudi¹, Kevan Jacobson¹, Bruce A. Vallance¹

¹Division of Gastroenterology, BC Children's Hospital

Citrobacter rodentium infection provides a valuable model to study enteric bacterial infections as well as host immune responses and colitis in mice. This protocol outlines the measurement of barrier integrity, pathogen load and histological damage allowing for the thorough characterization of pathogen and host contributions to murine infectious colitis.

Medicine

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

Vijay Morampudi^*1, Ganive Bhinder^*1, Xiujuan Wu¹, Chuanbin Dai¹, Ho Pan Sham¹, Bruce A. Vallance¹, Kevan Jacobson¹

¹Division of Gastroenterology, BC Children's Hospital

DNBS/TNBS induced colitis offers an alternative and inexpensive method to study the pathobiology of inflammatory bowel disease. The protocol discussed in this paper outlines the successful application of DNBS to induce colitis in mice and rats and allows one to thoroughly study host-mediated intestinal responses.