BC Children's Hospital
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis
Ganive Bhinder 1, Ho Pan Sham 1, Justin M. Chan 1, Vijay Morampudi 1, Kevan Jacobson 1, Bruce A. Vallance 1
1Division of Gastroenterology, BC Children's Hospital
Citrobacter rodentium infection provides a valuable model to study enteric bacterial infections as well as host immune responses and colitis in mice. This protocol outlines the measurement of barrier integrity, pathogen load and histological damage allowing for the thorough characterization of pathogen and host contributions to murine infectious colitis.
Medicine
DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
Vijay Morampudi *1, Ganive Bhinder *1, Xiujuan Wu 1, Chuanbin Dai 1, Ho Pan Sham 1, Bruce A. Vallance 1, Kevan Jacobson 1
1Division of Gastroenterology, BC Children's Hospital
DNBS/TNBS induced colitis offers an alternative and inexpensive method to study the pathobiology of inflammatory bowel disease. The protocol discussed in this paper outlines the successful application of DNBS to induce colitis in mice and rats and allows one to thoroughly study host-mediated intestinal responses.
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