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Helmholtz-Zentrum Berlin

3 ARTICLES PUBLISHED IN JoVE

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Biochemistry

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
Christian G. Feiler 1, Dirk Wallacher 2, Manfred S. Weiss 1
1Macromolecular Crystallography (HZB-MX), Helmholtz-Zentrum Berlin, 2Department Sample Environment, Helmholtz-Zentrum Berlin

A novel sample holder for macromolecular X-ray crystallography along with a suitable handling protocol is presented. The system allows crystal growth, crystal soaking and in situ diffraction data collection at both, ambient and cryogenic temperature without the need of any crystal manipulation or mounting.

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Biochemistry

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin
Jan Wollenhaupt 1, Tatjana Barthel 1,2, Gustavo M. A. Lima 3, Alexander Metz 4, Dirk Wallacher 5, Elmir Jagudin 3, Franziska U. Huschmann 1,4, Thomas Hauß 1, Christian G. Feiler 1, Martin Gerlach 1, Michael Hellmig 1, Ronald Förster 1, Michael Steffien 1, Andreas Heine 4, Gerhard Klebe 4, Uwe Mueller 1, Manfred S. Weiss 1
1Macromolecular Crystallography, Helmholtz-Zentrum Berlin, 2Structural Biochemistry Group, Institute for Chemistry and Biochemistry, Freie Universität Berlin, 3BioMAX, MAX IV Laboratory, 4Drug Design Group, Institute of Pharmaceutical Chemistry, Philipps-Universität Marburg, 5Department Sample Environment, Helmholtz-Zentrum Berlin

Crystallographic fragment screening at the Helmholtz-Zentrum Berlin is performed using a workflow with dedicated compound libraries, crystal handling tools, fast data collection facilities and largely automated data analysis. The presented protocol intends to maximize the output of such experiments to provide promising starting points for downstream structure-based ligand design.

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iNEXT-Discovery and Instruct-ERIC: Integrating High-End Services for Translational Research in Structural Biology
Hans Wienk 1, Lucia Banci 2, Susan Daenke 3, Eva Pereiro 4, Harald Schwalbe 5, David Ian Stuart 6, Manfred S. Weiss 7, Anastassis Perrakis 1
1Division of Biochemistry and Oncode Institute, The Netherlands Cancer Institute, Amsterdam, 2CERM/CIRMMP, University of Florence, 3Instruct-ERIC, Oxford University, 4MISTRAL beamline, Alba Light Source, 5Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, 6Division of Structural Biology, Oxford University, 7Macromolecular Crystallographyy, Helmholtz-Zentrum Berlin

iNEXT-Discovery and Instruct-ERIC: Integrating High-End Services for Translational Research in Structural Biology

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