VA Connecticut Healthcare Systems

4 ARTICLES PUBLISHED IN JoVE

Medicine

Technical Aspects of the Mouse Aortocaval Fistula

Kota Yamamoto^1,2, Xin Li^1,3, Chang Shu³, Tetsuro Miyata², Alan Dardik^1,4

¹The Department of Surgery and the Interdepartmental Program in Vascular Biology and Therapeutics, Yale University, ²Department of Vascular Surgery, The University of Tokyo, ³Department of Vascular Surgery, Central South University, ⁴VA Connecticut Healthcare Systems

The goal is to produce an arteriovenous fistula that is simple and reproducible. This method does not use sutures or glue adhesive. Therefore the samples can be used with the least amount of foreign materials for analysis.

Medicine

Intraluminal Drug Delivery to the Mouse Arteriovenous Fistula Endothelium

Takuya Hashimoto^1,2,3, Kota Yamamoto^1,2,3, Trenton Foster¹, Hualong Bai¹, Kunihiro Shigematsu⁴, Alan Dardik^1,3

¹Department of Surgery and the Vascular Biology and Therapeutics Program, Yale University, ²Department of Vascular Surgery, University of Tokyo, ³Department of Vascular Surgery, VA Connecticut Healthcare Systems, ⁴Department of Vascular Surgery, International University of Health and Welfare Mita Hospital

After puncturing the aorta through the inferior vena cava (IVC) to create an aorto-caval fistula in the mouse, solution containing a drug is infused into the IVC via the same needle, followed by incubation. This method enables more robust drug delivery to the venous endothelium compared to the external route.

Medicine

Patch Angioplasty in the Rat Aorta or Inferior Vena Cava

Hualong Bai^1,2,3,4,5, Xin Li⁶, Takuya Hashimoto^1,2,5, Haidi Hu^1,2,5, Trenton R. Foster^1,2,5, Jesse J. Hanisch^1,2,5, Jeans M. Santana^1,2,5, Alan Dardik^1,2,5

¹Department of Surgery, Yale University, ²Vascular Biology and Therapeutics Program, Yale University, ³Department of Vascular Surgery, First Affiliated Hospital of Zhengzhou University, ⁴Basic Medical College of Zhengzhou University, ⁵VA Connecticut Healthcare Systems, West Haven, CT, ⁶Department of Vascular Surgery, Xiangya Second Hospital of Central South University, Changsha, China

We have established a model of pericardial patch angioplasty that can be used in either small-diameter veins or arteries. This model can be used to compare venous and arterial neointimal hyperplasia formation.

Medicine

Murine Model of Central Venous Stenosis using Aortocaval Fistula with an Outflow Stenosis

Toshihiko Isaji^1,2,3, Shun Ono^1,2,4,5, Takuya Hashimoto^1,2,3, Kota Yamamoto^1,2,3, Ryosuke Taniguchi^1,2,3, Haidi Hu^1,2, Tun Wang^1,2, Jun Koizumi⁴, Toshiya Nishibe⁵, Katsuyuki Hoshina³, Alan Dardik^1,2,6

¹Department of Surgery, Yale University, ²Vascular Biology and Therapeutics Program, Yale University, ³Department of Vascular Surgery, University of Tokyo, ⁴Department of Diagnostic Radiology, Tokai University School of Medicine, ⁵Department of Cardiovascular Surgery, Tokyo Medical University, ⁶Department of Vascular Surgery, VA Connecticut Healthcare Systems

An aortocaval fistula was created by puncturing the murine infra-renal aorta through both walls into the inferior vena cava and was followed by creation of a stenosis in its outflow via partial ligation of the inferior vena cava. This reproducible model can be used to study central venous stenosis.