Max Plank Institute of Molecular Plant Physiology

1 ARTICLES PUBLISHED IN JoVE

Biology

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Witold G. Szymanski¹, Sylwia Kierszniowska¹, Waltraud X. Schulze^1,2

¹Signaling Proteomics, Max Plank Institute of Molecular Plant Physiology, ²Department of Plant Systems Biology, University of Hohenheim

Here we describe a robust method for the fractionation of plant plasma membranes into detergent resistant and detergent soluble membranes based on a mixture of unlabeled and in vivo fully ¹⁵N labeled Arabidopsis thaliana cell cultures. The procedure is applied for comparative proteomic studies to understand signaling processes.