Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell CulturesWitold G. Szymanski 1, Sylwia Kierszniowska 1, Waltraud X. Schulze 1,2
1Signaling Proteomics, Max Plank Institute of Molecular Plant Physiology, 2Department of Plant Systems Biology, University of Hohenheim
Here we describe a robust method for the fractionation of plant plasma membranes into detergent resistant and detergent soluble membranes based on a mixture of unlabeled and in vivo fully 15N labeled Arabidopsis thaliana cell cultures. The procedure is applied for comparative proteomic studies to understand signaling processes.
