The MultiBac Protein Complex Production Platform at the EMBLImre Berger 1, Frederic Garzoni 1, Maxime Chaillet 1, Matthias Haffke 1, Kapil Gupta 1, Alice Aubert 1
1EMBL Grenoble Outstation and Unit of Virus Host Cell Interactions (UVHCI) UMR5322
Protein complexes catalyze key cellular functions. Detailed functional and structural characterization of many essential complexes requires recombinant production. MultiBac is a baculovirus/insect cell system particularly tailored for expressing eukaryotic proteins and their complexes. MultiBac was implemented as an open-access platform, and standard operating procedures developed to maximize its utility.