An erratum was issued for: In situ TEM of Biological Assemblies in Liquid. The Representative Results and References sections were updated.
Here we describe a procedure to image viral complexes in liquid at nanometer resolution using a transmission electron microscope.
Here protocols are described to prepare virus assemblies suitable for liquid-EM and cryo-EM analysis at the nanoscale using transmission electron microscopy.
Here, we present a protocol to utilize machine-vision software to stabilize dynamic processes during TEM imaging, while concurrently indexing multiple streams of metadata to each image into a navigable timeline. We demonstrate how this platform enables automated calibration and mapping of the electron dose over the course of an experiment.
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