JoVE Logo

Sign In

Helmholtz Center for Infection Research

6 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy
Mike Hasenberg 1, Anja Köhler 1, Susanne Bonifatius 1, Andreas Jeron 2, Matthias Gunzer 1
1Institute for Molecular and Clinical Immunology, Otto-von-Guericke University Magdeburg, 2Department of Immunoregulation, Helmholtz Center for Infection Research

We show, how to use 2-photon microscopy for the observation of the dynamics of neutrophil granulocytes in infected lungs while they phagocytose pathogens or produce neutrophil extracellular traps (NETs).

image

Immunology and Infection

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
Marcus Gereke 1,2, Andrea Autengruber 1,2, Lothar Gröbe 3, Andreas Jeron 2, Dunja Bruder 1,2, Sabine Stegemann-Koniszewski 1
1Research Group Immune Regulation, Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology, Otto-von-Guericke University , 3Department of Experimental Immunology, Helmholtz Centre for Infection Research

We describe the rapid isolation of primary murine type II alveolar epithelial cells (AECII) by flow cytometric negative selection. These AECII show high viability and purity and are suitable for a wide range of functional and molecular studies regarding their role in respiratory conditions such as autoimmune or infectious diseases.

image

JoVE Journal

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
Tim Pieters 1,2,3,4, Lieven Haenebalcke 1,2, Kenneth Bruneel 1,2,4, Niels Vandamme 1,2,4, Tino Hochepied 1,2, Jolanda van Hengel 5, Dagmar Wirth 6, Geert Berx 1,2,4, Jody J. Haigh 7, Frans van Roy 1,2,4, Steven Goossens 1,2,3,4
1Department of Biomedical Molecular Biology, Ghent University, 2Inflammation Research Center, VIB, 3Center for Medical Genetics, Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology, Monash University and Alfred Health Alfred Centre

Proteins often contain multiple domains that can exert different cellular functions. Gene knock-outs (KO) do not consider this functional diversity. Here, we report a recombination-mediated cassette exchange (RMCE)-based structure-function approach in KO embryonic stem cells that allows for the molecular dissection of various functional domains or variants of a protein.

image

Neuroscience

Optimized Management of Endovascular Treatment for Acute Ischemic Stroke
Katharina Schregel 1,2, Daniel Behme 1, Ioannis Tsogkas 1, Michael Knauth 1, Ilko Maier 3, André Karch 4, Rafael Mikolajczyk 4,5, Mathias Bähr 3, Jörn Schäper 6, José Hinz 6, Jan Liman 3, Marios-Nikos Psychogios 1
1Institute of Neuroradiology, University Medical Center Goettingen, 2Department of Radiology, Brigham and Women's Hospital and Harvard Medical School, 3Department of Neurology, University Medical Center Goettingen, 4Department of Epidemiology, Helmholtz Center for Infection Research, 5Institute of Medical Epidemiology, Biostatistics and Informatics, Martin-Luther-University Halle-Wittenberg, 6Department of Anesthesiology, University Medical Center Goettingen

The outcome of patients with acute ischemic stroke depends on swift restoration of cerebral blood flow. This protocol aims at optimizing the management of such patients by minimizing peri-procedural timings and rendering the time from hospital admission to reperfusion as short as possible.

image

Immunology and Infection

Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow
Hilger Jagau 1,2, Ina-Kristin Behrens 1,3, Michael Steinert 1,4, Simone Bergmann 1
1Institut für Mikrobiologie, Technische Universität Braunschweig, 2Devision of Infection Medicine, Department of Clinical Science, Lund University, 3Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Ludwig Maximilians University, 4Department of Molecular Infection Biology, Helmholtz Center for Infection Research

This study describes the microscopic monitoring of pneumococcus adherence to von Willebrand factor strings produced on the surface of differentiated human primary endothelial cells under shear stress in defined flow conditions. This protocol can be extended to detailed visualization of specific cell structures and quantification of bacteria by applying differential immunostaining procedures.

image

Developmental Biology

Development of a Larval Zebrafish Infection Model for Clostridioides difficile
Junkai Li 1, Can M. Ünal 2, Kazuhiko Namikawa 1, Michael Steinert 3,4,5, Reinhard W. Köster 1
1Division of Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, 2Department of Molecular Biotechnology, Turkish-German University, 3Institut für Mikrobiologie, Technische Universität Braunschweig, 4Braunschweig Integrated Centre of Systems Biology, 5Helmholtz Centre for Infection Research

Presented here is a safe and effective method to infect zebrafish larvae with fluorescently labeled anaerobic C. difficile by microinjection and noninvasive microgavage.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved