Stevens Institute of Technology

7 ARTICLES PUBLISHED IN JoVE

Biology

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

Wei Hu^*1, Amin Tamadon^*1, Aaron J.W. Hsueh², Yi Feng¹

¹Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences; Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine, Fudan University, ²Program of Reproductive and Stem Cell Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford University

Here we present an adaptation of the passive CLARITY and 3D reconstruction method for visualization of the ovarian vasculature and follicular capillaries in intact mouse ovaries.

Behavior

Characterization of the Sense of Agency over the Actions of Neural-machine Interface-operated Prostheses

Jonathon S. Schofield¹, Courtney E. Shell¹, Zachary C. Thumser^1,2, Dylan T. Beckler¹, Raviraj Nataraj³, Paul D. Marasco^1,4

¹Laboratory for Bionic Integration, Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, ²Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, ³Department of Biomedical Engineering, Stevens Institute of Technology, ⁴Advanced Platform Technology Center, Louis Stokes Cleveland Department of Veterans Affairs Medical Center

Here we present a protocol which characterizes the sense of agency developed over the control of sensate virtual or robotic prosthetic hands. Psychophysical questionnaires are employed to capture the explicit experience of agency, and time interval estimates (intentional binding) are employed to implicitly measure the sense of agency.

Chemistry

Light-driven Molecular Motors on Surfaces for Single Molecular Imaging

Jiawen Chen¹, Jérôme Vachon¹, Ben L. Feringa¹

¹Stratingh Institute for Chemistry, University of Groningen

The manuscript describes how to synthesize and graft a molecular motor on surfaces for single molecular imaging.

Immunology and Infection

Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell (CB-SC)-Derived Exosomes

Wei Hu^1,2, Xiang Song¹, Haibo Yu¹, Jingyu Sun², Yong Zhao¹

¹Center for Discovery and Innovation, Hackensack Meridian Health Center, ²Department of Chemistry and Chemistry Biology, Stevens Institute of Technology

Exosome application is an emerging tool for drug development and regenerative medicine. We establish an exosome isolation protocol with high purity to isolate exosomes from novel identified stem cells called CB-SC for mechanistic studies. We also coculture CB-SC-derived exosomes with human monocytes, leading to their differentiation into phenotypically distinct macrophages.

Biology

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

Christina Li¹, Jeel Shah¹, Kylee Wrath¹, Dahlia Matouba¹, Connor Mills¹, Kishore Punnath², Ansu Perekatt¹

¹Department of Biology and Chemical Biology, Stevens Institute of Technology, ²Department of Pharmacology, Pennsylvania State University College of Medicine

The procedure describes isolation of the villi from the mouse intestinal epithelium undergoing dedifferentiation to determine their organoid forming potential.

Developmental Biology

Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

Wenyi Wang¹, Tao Kang¹, Lin Bai¹, Wei Hu², Yayoi Obata³, Jianzhen Li¹

¹College of Life Sciences, Northwest Normal University, ²State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, ³Department of Bioscience, Tokyo University of Agriculture

Here, we present a simple method for separating follicular cells and oocytes in zebrafish ovarian follicles, which will facilitate investigations of ovarian development in zebrafish.

Bioengineering

Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue

Mohammad Mir¹, Jiawen Chen¹, Meghan R. Pinezich¹, John D. O’Neill³, Brandon A. Guenthart⁴, Gordana Vunjak-Novakovic², Jinho Kim¹

¹Department of Biomedical Engineering, Stevens Institute of Technology, ²Department of Biomedical Engineering, Columbia University, ³Department of Cell Biology, State University of New York Downstate Medical Center, ⁴Department of Cardiothoracic Surgery, Stanford University

The protocol describes an imaging-enabled bioreactor that allows the selective removal of the endogenous epithelium from the rat trachea and homogenous distribution of exogenous cells on the lumen surface, followed by long-term in vitro culture of the cell-tissue construct.