Construction of Gradient Index (GRIN) Lens-Based Imaging Device
2:25
Imaging-Guided Removal of the Trachea Epithelium
5:51
Homogeneous Distribution of Exogenous Cells onto the Denuded Tracheal Lumen
8:23
Results: Imaging-Guided Bioreactors for the Generation of Functional In Vitro Airway Tissues
10:18
Conclusion
Transcript
This protocol allows in vitro cultivation and direct microscopic visualization of the isolated airway tissues during different tissue manipulation procedures, such as de-epithelization and airway tissue regeneration. The in situ imaging proposed i
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The protocol describes an imaging-enabled bioreactor that allows the selective removal of the endogenous epithelium from the rat trachea and homogenous distribution of exogenous cells on the lumen surface, followed by long-term in vitro culture of the cell-tissue construct.