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Friedrich Schiller University Jena

5 ARTICLES PUBLISHED IN JoVE

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Neuroscience

In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves
Alexander Schulz 1, Christian Walther 2, Helen Morrison 1, Reinhard Bauer 3
1Leibniz Institute for Age Research, Fritz Lipmann Institute, 2Friedrich Schiller University Jena, 3Institute of Molecular Cell Biology & Center for Sepsis Control and Care (CSCC) Jena University Hospital, Friedrich Schiller University Jena

Measurements of nerve conduction properties in vivo exemplify a powerful tool to characterize various animal models of neuromuscular diseases. Here, we present an easy and reliable protocol by which electrophysiological analysis on sciatic nerves of anesthetized mice can be performed.

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Immunology and Infection

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection
Marten B. Maeß 1, Berith Wittig 1, Stefan Lorkowski 1
1Institute of Nutrition, Friedrich Schiller University Jena

This protocol presents an efficient and reliable method to transfect human THP-1 macrophages with siRNA or plasmid DNA by electroporation with high transfection efficiency while maintaining high cell vitality and full macrophage capacity for differentiation and polarization.

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Chemistry

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
Katharina Grosser 1,2, Nicole M. van Dam 1,2
1German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, 2Institute of Ecology, Friedrich Schiller University Jena

Here, we describe in great detail an established and robust protocol for the extraction of glucosinolates from ground plant materials. After an on-column sulfatase treatment of the extracts, the desulfoglucosinolates are eluted and analyzed by high-pressure liquid chromatography.

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Behavior

Eye Tracking During Visually Situated Language Comprehension: Flexibility and Limitations in Uncovering Visual Context Effects
Camilo Rodriguez Ronderos 1, Katja Münster 1, Ernesto Guerra 2, Helene Kreysa 3, Alba Rodríguez 1,4, Julia Kröger 4, Thomas Kluth 4, Michele Burigo 4, Dato Abashidze 5, Eva Nunnemann 4, Pia Knoeferle 1,6,7
1Institute of German Studies and Linguistics, Humboldt-Universität zu Berlin, 2Center for Advanced Research in Education – CIAE, Universidad de Chile, 3Institute of Psychology, Friedrich Schiller University Jena, 4Cognitive Interaction Technology Excellence Cluster (CITEC), Faculty of Linguistics and Literature, Bielefeld University, 5Department of Education, Concordia University, 6Berlin School of Mind and Brain, 7Einstein Center for Neurosciences Berlin

The present article reviews an eye-tracking methodology for studies on language comprehension. To obtain reliable data, key steps of the protocol must be followed. Among these are the correct set-up of the eye tracker (e.g., ensuring good quality of the eye and head images) and accurate calibration.

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Immunology and Infection

Immunocompetent Alveolus-on-Chip Model for Studying Alveolar Mucosal Immune Responses
Hristina Koceva 1, Mona Amiratashani 1, Knut Rennert 2, Alexander S. Mosig 1,3,4
1Institute of Biochemistry II, Jena University Hospital, 2Dynamic42 GmbH, 3Center for Sepsis Control and Care, Jena University Hospital, 4Jena School of Microbial Communication, Friedrich Schiller University Jena

Lung-on-chip models surpass traditional 2D cultures by mimicking the air-liquid interface and endothelial cell perfusion, simulating blood flow and nutrient exchange crucial for lung physiology studies. This enhances lung research relevance, offering a dynamic, physiologically accurate environment to advance the understanding and treatment of respiratory infections.

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