National Center for Global Health and Medicine

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Hironori Nishitsuji¹, Hiromi Yamamoto¹, Ritsuko Shiina¹, Keisuke Harada², Saneyuki Ujino¹, Kunitada Shimotohno¹

¹Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, ²Central Pharmaceutical Research Institute, Japan Tobacco Inc.

Here we describe a newly developed hepatitis B virus (HBV) reporter system to monitor the early stages of the HBV life cycle. This simplified in vitro system will aid in the screening of anti-HBV agents using a high-throughput strategy.

Bioengineering

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Hiroshi Kobayashi¹, Keiyo Takubo¹

¹Department of Stem Cell Biology, Research Institute, National Center for Global Health and Medicine

This protocol enables the maintenance of quiescent human hematopoietic stem cells in vitro by mimicking the bone marrow microenvironment using abundant fatty acids, cholesterol, lower concentrations of cytokines, and hypoxia.